- Letter to the Editor
- Open Access
Progression patterns in monoclonal gammopathy of undetermined significance and multiple myeloma outcome: a cohort study in 42 patients
Experimental Hematology & Oncology volume 11, Article number: 8 (2022)
Follow-up of low-risk monoclonal gammopathy of undetermined significance (MGUS) is debated as multiple myeloma (MM) progression risk is low. Worse MM outcome was reported for patients followed for low-risk MGUS, possibly due to less optimal follow-up. However, it is unknown whether progressing low-risk MGUS is associated with aggressive tumor behavior. Understanding these patterns is crucial for MGUS management. Here, we investigated whether progression from low-risk MGUS is associated with worse MM outcome in patients who had no MGUS follow-up before myeloma diagnosis. We retrospectively determined the MGUS status in repeated pre-diagnostic blood samples prospectively collected from 42 myeloma patients in median 11.6 years (first sample) and 3.3 years (repeated sample) before myeloma diagnosis. At first pre-diagnostic blood draw, 12 had low-risk (defined by an immunoglobulin [Ig] G monoclonal [M] spike < 15 g/L and a normal free light-chain ratio) and 30 had MGUS of other risk. MM bone disease was more common in patients with low-risk MGUS at first blood draw (67% vs. 30%, P = 0.041). Median survival since myeloma diagnosis was worse in low-risk than other MGUS at first blood draw (2.3 vs. 7.5 years, P = 0.004). Modest progression was observed between first and repeated blood draw for the majority of low-risk MGUS as 67% remained as low- or low-intermediate-risk MGUS at repeated blood draw. Our study, albeit limited by its small size, indicates that progression from low-risk MGUS is associated with worse MM outcome regardless of MGUS follow-up. Although further investigation is needed, progressing low-risk MGUS could belong to a group of aggressive tumors with progression that is difficult to predict.
To the editor
Multiple myeloma (MM) is preceded by monoclonal gammopathy of undetermined significance (MGUS) [1, 2]. Guidelines recommend following MGUS according to MM progression risk . Follow-up of low-risk MGUS is debated as progression risk is low (5% at 20 years) . Studies evaluating MGUS follow-up indicate worse MM outcome in patients followed for low-risk MGUS, possibly due to less optimal follow-up [5, 6]. However, it is unknown whether progressing low-risk MGUS is associated with aggressive tumor behavior. Understanding these patterns is important for MGUS management . Therefore, the association between progressing low-risk MGUS and MM outcome needs further study. We investigated whether progression from low-risk MGUS is associated with worse MM outcome in patients who had no MGUS follow-up before myeloma diagnosis. The MGUS status was determined retrospectively in repeated pre-diagnostic blood samples of 42 myeloma patients.
The Umeå University review board approved this study using samples from the Northern Sweden Health and Disease Study, a large prospective cohort. Linkage to the Swedish Cancer Registry facilitated identification of myeloma patients with a first and repeated pre-diagnostic blood sample before myeloma diagnosis. We could study natural progression patterns in relation to MM outcome because 42 had detectable MGUS (protein and immunofixation electrophoresis and free light-chain assays) in both pre-diagnostic samples without MGUS follow-up before myeloma diagnosis. Kaplan–Meier plots and multivariable Cox regression were used to study overall survival (Additional file 1).
The first pre-diagnostic blood sample was donated in November 1986 and the last follow-up since myeloma diagnosis was in February 2021 providing a 19-year study duration in median. Median times since first and repeated pre-diagnostic blood draw to myeloma diagnosis were 11.6 and 3.3 years (Table 1). At first pre-diagnostic blood draw, 12 had low-risk (defined by immunoglobulin [Ig] G monoclonal [M] spike < 15 g/L and normal free light-chain ratio) and 30 had MGUS of any other risk category (i.e., low-intermediate-risk, high-intermediate-risk, high-risk, or light-chain MGUS) (Table 1). Male sex was more common in patients with low-risk MGUS at first blood draw. Other characteristics, including age, diagnosis year, comorbidities, myeloma therapy, and access to novel drugs, were similar in both groups (Table 1, Additional File 2: Table S1–2).
At myeloma diagnosis, 83% vs. 57% had symptomatic MM in patients who had low-risk vs. other MGUS, respectively, at first pre-diagnostic blood draw (P = 0.158) (Table 1). Excluding light-chain myeloma (N = 8), formal statistical significance was reached (83% vs. 41% [9 of 22], P = 0.030). At myeloma diagnosis, bone disease (osteolytic lesions and/or vertebral compression fractures due to MM) was more common in low-risk vs. other MGUS at first blood draw (P = 0.041; Table 1). This was pronounced excluding light-chain myeloma (P = 0.008). Imaging along conventional skeletal surveys was similarly used in both groups (Table 1). In low-risk vs. other MGUS, median survival since myeloma diagnosis was 2.3 years vs. 7.5 years (Fig. 1A). Results were similar for survival since therapy start and in multivariable analyses (Fig. 1A, B). Sex was not associated with bone disease and survival. The results were confirmed in several sensitivity analyses (Additional file 1).
We compared MM progression trajectories in patients who had low-risk vs. other MGUS (restricted to IgG isotype for better comparison) at first pre-diagnostic blood draw. At repeated pre-diagnostic blood draw, progression to smoldering multiple myeloma (M spike ≥ 30 g/L) was observed in 8% (1 of 12) in low-risk vs. 20% (3 of 15) in other MGUS (P = 0.605). More patients with low-risk MGUS at first pre-diagnostic blood draw had lower MGUS risk (low- or low-intermediate-risk) at repeated blood draw compared to other MGUS (67% [8 of 12] vs. 27% [4 of 15], P = 0.057). This was pronounced excluding four patients who did not progress to symptomatic MM (all had other MGUS at first blood draw; 67% vs. 9% [1 of 11], P = 0.009). These observations could indicate a more rapid progression process in low-risk MGUS closer to MM initiation. Investigating this, we plotted M spikes in both groups. M spike trajectories were visually largely similar in both groups with some patients experiencing rapid clonal evolution as indicated by fast increasing paraprotein levels (Fig. 1C, D). The annual median M spike increase since repeated pre-diagnostic blood draw was 6.0 g/L in low-risk and 2.2 g/L in other MGUS (P = 0.13) (Fig. 1C, D, Additional File 2: Table S3).
Our study, which included data collected over 19 years in median with 81% treated using novel drugs and overall survival comparable to other studies , shows that progression from low-risk MGUS is associated with worse MM outcome. These results agree with previous data [5, 6]; however, we found progressing low-risk MGUS associated with worse MM outcome in patients who had no MGUS follow-up before myeloma diagnosis. Thus, we speculate that progressing low-risk MGUS could belong to a group of more aggressive tumors. Biological mechanisms for this putative association are unclear. Interestingly, substantial genomic differences in patients with stable and progressive myeloma precursor condition have been observed  with distinct genomic patterns of progression (“static progression” vs. “spontaneous evolution”) in patients who progressed from SMM to MM . Consistent with recent data , progression would have been difficult to predict in many of the low-risk patients as 67% remained low- or low-intermediate-risk MGUS at repeated blood draw (donated in median 7.5 years after the first sample). These observations illustrate limitations of the current MGUS stratification: (i) reduced sensitivity in the accurate identification of low-risk MGUS and (ii) current biomarkers do not predict the biological behavior of the later diagnosed tumor. Accurate early prediction of disease progression and/or aggressive tumor behavior could facilitate the identification of patients potentially benefiting from early therapeutic intervention such as currently under evaluation in high-risk MGUS and low-risk SMM .
We speculate that while low-risk MGUS patients are less likely to develop MM, there is a subset of these patients who will progress and, importantly, in case of progression belong to a group of more aggressive tumors. As this study has a small sample size with cytogenetic information only available in 17% of the patients and the most recent IMWG criteria  were not applicable, the results require further investigation. Until ongoing studies provide answers , our data stress the need for improved MGUS stratification based on specific molecular features rather than biomarkers largely reflective of tumor burden . Investigation of microenvironmental differences in prospective blood samples among stable and progressing MGUS could help (i) increase the understanding of underlying extrinsic factors in MM progression and (ii) identify useful biomarkers.
Availability of data and materials
All data are available in the manuscript or the additional file materials.
Autologous stem cell transplant
Eastern Cooperative Oncology Group
International Myeloma Working Group
International Staging System
Monoclonal gammopathy of undetermined significance
- M (spike):
Smoldering multiple myeloma
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We wish to thank the Biobank Research Unit at Umeå University and the County Council of Västerbotten for providing data and samples and acknowledge the contribution from Biobank Sweden, supported by the Swedish Research Council (VR 2017-00650).
The Cancer Research Foundation in Northern Sweden (FS), the regional agreement between Umeå University and Västerbotten County Council (ALF) (FS), and the Faculty of Medicine at Umeå University (FS). The funders had no role in study design, collection, analysis, and interpretation of data and in writing the manuscript.
Ethics approval and consent to participate
All participants provided written consent at enrollment of the Northern Sweden Health and Disease Study to participate in future research projects. The present study was approved by the ethical review board at Umeå University (reference no. 2017/242-31).
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The authors declare no potential conflicts of interest.
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Additional file 1:
Patients and methods.
Additional file 2:
Table S1. Comorbidities in patients who had low-risk vs. other MGUS at first pre-diagnostic blood draw. Table S2. Myeloma treatment in patients who had low-risk vs. other MGUS at first pre-diagnostic blood draw. Table S3. M spike concentrations in low-risk vs. other MGUS of IgG isotype (for better comparison) at first blood draw.
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Tahiru, W., Izarra Santamaria, A., Hultdin, J. et al. Progression patterns in monoclonal gammopathy of undetermined significance and multiple myeloma outcome: a cohort study in 42 patients. Exp Hematol Oncol 11, 8 (2022). https://doi.org/10.1186/s40164-022-00259-0
- Multiple myeloma
- Myeloma outcome
- Aggressive myeloma
- Low-risk MGUS
- MGUS follow-up
- MGUS progression
- Prospective blood samples