Skip to main content

Etanercept as a new therapeutic option for cytokine release syndrome following chimeric antigen receptor T cell therapy


Cytokine release syndrome (CRS) is the most common toxicity induced by chimeric antigen receptor (CAR) T cell therapy. At present, anti-IL-6 agents including tocilizumab and siltuximab have been applied in the treatment of CRS. However, tocilizumab and siltuximab are expensive and some patients fail to respond to anti-IL-6 therapy, which urges the need for new drugs. In clinical practice, we found some patients with multiple myeloma developed markedly increased levels of tumor necrosis factor (TNF)- α during the CRS period after anti-BCMA CAR T cell infusion. Here we present the successful use of TNF-α inhibitor (etanercept) to cure CRS in three patients. The introduction of etanercept did not alter patients' response to CAR T cell therapy and no adverse event was observed directly related to the administration of etanercept. Furthermore, in vitro experiments confirmed that etanercept did not affect the proliferation and effector function of CAR T cells. Our results indicate that etanercept could be considered as a treatment option for CRS in patients with significantly elevated TNF-α levels.

To the Editor,

Over the past decade, chimeric antigen receptor (CAR) T cell therapy has brought hope to patients with relapsed/refractory multiple myeloma (R/R MM), but toxicities such as cytokine release syndrome (CRS) have emerged as new challenges [1]. The CARTOX Working Group and the NCCN guidelines both recommend anti-IL-6 agents (tocilizumab and siltuximab) and corticosteroids as treatments for CRS [2, 3]. However, improved treatment for CRS remains an unmet clinical need, because the existing drugs are not always sufficiently effective. IL-1R antagonist (anakinra) [4], GM-CSF inhibition (lenzilumab) [5], and plasma exchange [6] have been reported as managements for CRS. Moreover, Lee, et al. [7]. successfully used TNF-α inhibitor (etanercept) to treat grade 3 CRS in a 19-year-old female patient with relapsed Hodgkin lymphoma. This was, however, not sufficient to confirm the therapeutic effects of etanercept because she was treated with etanercept and methylprednisolone simultaneously.

Eight patients with R/R MM were enrolled in our study and received LCAR-B38M (anti-BCMA CAR T cells) infusion from March 2017 to March 2020. The clinical characteristics of patients and details of CAR T cell therapy are summarized in Table 1. Fifty-four cytokines were monitored before and after LCAR-B38M infusion by Luminex in all the patients and the detailed results are reported in Additional file 1: Table S1. We found a significant increase in IL-6, TNF-α, IL-10 and TGF-α during the clinical CRS period. More interestingly, Patient 1, Patient 4 and Patient 8 exhibited markedly elevated levels of TNF-α, which was the reason for using etanercept to treat CRS in these patients.

Table 1 Clinical data of patients and details of LCAR-B38M therapy

Patient 1 presented with a fever of up to 39.0℃ on day 7 and was treated with paracetamol. However, his body temperature reached 39.4℃ on the second day and he received a subcutaneous injection of etanercept (25 mg) on day 8 (Fig. 1b). Subsequently, his temperature gradually returned to normal. Patient 4 presented with fever on day 8 and experienced neutropenia (grade 3), increased serum aspartate aminotransferase (grade 3), hypotension (grade 3) and arthralgia (grade 3). On days 10 and 11, he received intravenous tocilizumab (240 mg on day 10 and 240 mg twice a day on day 11) and additional supportive care. However, his symptoms did not improve despite the repeated use of tocilizumab; therefore, we used etanercept (50 mg) on day 11 (Fig. 1c). He responded promptly to this treatment and recovery occurred gradually. Patient 8 developed grade 1 CRS on day 6, which was managed with antipyretics. However, he again developed a fever of up to 38.5℃ on day 14. No evidence of severe infection was present. We considered that the re-emergence of fever was also associated with CRS because the serum IL-6 level also increased. Notably, his serum TNF-α level was more than 30 times higher than the baseline. Therefore, etanercept (25 mg) was given on day 17 and symptoms did not recur again (Fig. 1d).

Fig. 1

Clinical efficacy of etanercept for cytokine release syndrome and in vitro effects of etanercept on CAR T cells. a Maximum temperature for each day of the 3 patients after LCAR-B38M infusion. Arrows indicate the day of etanercept administration. b–d IL-6 and TNF-α levels before and after LCAR-B38M infusion in each patient. Cytokine levels are expressed as log10 pg/mL. The values less than the detection limit of serum cytokines were defined as “0” in the statistical analyses. Arrows indicate the day of etanercept administration. e Etanercept does not affect CAR T cell proliferation. CAR T cells was treated with 10 μg/mL, 2 μg/mL, 0.4 μg/mL, 0.08 μg/mL, 0 μg/mL etanercept for 5 days. On day 1, day 3 and day 5, cells are collected for cell counting. ns, P > 0.05. f, g Etanercept does not affect CAR T cell effector function. CAR T cells or untransduced T cells (UNT) as effector cells are cocultured with RPMI8226-Luc cells or H929-Luc cells at the effector to target cell ratio of 4:1, 2:1 and 1:1 with or without 10 μg/mL etanercept. ns, P > 0.05

To our knowledge, this study is the first attempt to use etanercept monotherapy to treat CRS following CAR T cell therapy. All three patients achieved sCR and we did not observe adverse events directly related to administration of etanercept. Our findings were further supported by in vitro experiments demonstrating that etanercept did not affect CAR T cell proliferation or killing effects on MM cells (Fig. 1e–g). Specific details were provided in Additional file 1.

TNF-α, a major proinflammatory cytokine, is secreted by activated macrophages, monocytes and lymphocytes [8]. Significantly elevated levels of TNF-α have been reported in patients with acute lymphoblastic leukemia who received anti-CD19 CAR T cell infusion [9], which was consistent with our results. Furthermore, the peak level of TNF-α in the serum of patients was considered associated with the severity of CRS [9]. These findings suggest that TNF-α might play an important role in CRS. Etanercept has been widely used in the treatment of rheumatoid arthritis and its efficacy and safety are well established [10]. We suggest that etanercept can be used to manage CRS associated with CAR T cell therapy, and especially recommend for patients suffering from a rapid elevation of TNF-α. Of course, further studies are needed to confirm this suggestion.

Availability of data and materials

Data sharing is not applicable to our study.



Chimeric antigen receptor


Multiple myeloma




Cytokine release syndrome


Tumor necrosis factor


Stringent complete response


B cell maturation antigen.


  1. 1.

    Liu D, Zhao J. Cytokine release syndrome: grading, modeling, and new therapy. J Hematol Oncol. 2018;11:121.

    Article  Google Scholar 

  2. 2.

    Neelapu SS, Tummala S, Kebriaei P, Wierda W, Gutierrez C, Locke FL, et al. Chimeric antigen receptor T-cell therapy - assessment and management of toxicities. Nat Rev Clin Oncol. 2018;15:47–62.

    CAS  Article  Google Scholar 

  3. 3.

    Thompson JA, Schneider BJ, Brahmer J, Andrews S, Armand P, Bhatia S, et al. NCCN Guidelines Insights: Management of Immunotherapy-Related Toxicities, Version 1.2020. J Natl Compr Canc Netw. 2020;18:230–41.

  4. 4.

    Jatiani SS, Aleman A, Madduri D, Chari A, Cho HJ, Richard S, et al. Myeloma CAR-T CRS Management With IL-1R Antagonist Anakinra. Clin Lymphoma Myeloma Leuk. 2020;20:632–6.

    Article  Google Scholar 

  5. 5.

    Sterner RM, Sakemura R, Cox MJ, Yang N, Khadka RH, Forsman CL, et al. GM-CSF inhibition reduces cytokine release syndrome and neuroinflammation but enhances CAR-T cell function in xenografts. Blood. 2019;133:697–709.

    CAS  Article  Google Scholar 

  6. 6.

    Xiao X, He X, Li Q, Zhang H, Meng J, Jiang Y, et al. Plasma Exchange Can Be an Alternative Therapeutic Modality for Severe Cytokine Release Syndrome after Chimeric Antigen Receptor-T Cell Infusion: A Case Report. Clin Cancer Res. 2019;25:29–34.

    Article  Google Scholar 

  7. 7.

    Lee DW, Gardner R, Porter DL, Louis CU, Ahmed N, Jensen M, et al. Current concepts in the diagnosis and management of cytokine release syndrome. Blood. 2014;124:188–95.

    CAS  Article  Google Scholar 

  8. 8.

    Mitoma H, Horiuchi T, Tsukamoto H, Ueda N. Molecular mechanisms of action of anti-TNF-α agents - Comparison among therapeutic TNF-α antagonists. Cytokine. 2018;101:56–63.

    CAS  Article  Google Scholar 

  9. 9.

    Wang J, Mou N, Yang Z, Li Q, Jiang Y, Meng J, et al. Efficacy and safety of humanized anti-CD19-CAR-T therapy following intensive lymphodepleting chemotherapy for refractory/relapsed B acute lymphoblastic leukaemia. Br J Haematol. 2020;191:212–22.

    CAS  Article  Google Scholar 

  10. 10.

    Tracey D, Klareskog L, Sasso EH, Salfeld JG, Tak PP. Tumor necrosis factor antagonist mechanisms of action: a comprehensive review. Pharmacol Ther. 2008;117: 244–79.

Download references


We thank the patients and their families for participating in this research.


This work was supported by grants from the National Natural Science Foundation of China (No.81670199, No.81720108002), National Science and Technology Major Project (2018ZX09734007), and Jiangsu Province’s Medical Elite Program (ZDRCA2016015).

Author information




Conception and design: LC, JL. Development of methodology: LC, LZ. Acquisition of data: SW, JX, RZ, HZ, YW, LZ, Analysis and interpretation of data: LZ, SW, JX, RZ, HZ, YW, LZ. Writing, review, and/or revision of the manuscript: LC, LZ. Administrative, technical, or material support: YW, LZ, JL. Study supervision: LC. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Lijuan Chen.

Ethics declarations

Ethics approval and consent to participate

This study was approved by the institutional review boards of the First Affiliated Hospital of Nanjing Medical University Ethics Committee. Informed consent was obtained from all patients for the treatment protocol.

Consent for publication

Informed consent for research and publication from the patients was obtained.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1.

Additional tables.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, L., Wang, S., Xu, J. et al. Etanercept as a new therapeutic option for cytokine release syndrome following chimeric antigen receptor T cell therapy. Exp Hematol Oncol 10, 16 (2021).

Download citation


  • Tumor necrosis factor -α
  • Etanercept
  • Cytokine release syndrome
  • Chimeric antigen receptor T cell therapy
  • Multiple myeloma