Open Access

Acute lymphoblastic leukemia with e1a3 BCR/ABL fusion protein. A report of two cases

  • Bernardo López-Andrade1,
  • Francesca Sartori1,
  • Antonio Gutiérrez1, 5,
  • Lucia García1,
  • Vanesa Cunill3,
  • María Antonia Durán1,
  • Antonia Sampol1, 5,
  • Marta Bernués2,
  • Julio Iglesias3,
  • Rafael Ramos4,
  • Josep Lladó1,
  • María Sánchez1,
  • Juan Carlos Amat1 and
  • Jordi Martínez-Serra1, 5Email author
Contributed equally
Experimental Hematology & Oncology20165:21

https://doi.org/10.1186/s40164-016-0049-y

Received: 12 April 2016

Accepted: 6 July 2016

Published: 29 July 2016

Abstract

B Acute Lymphoblastic leukemia (B-ALL) with Philadelphia chromosome (Ph′) is a neoplasm of lymphoblast committed to the B cell lineage. The clinical presentation of B-ALL Ph′+ is similar to B-ALL, but is more common in adults than in children. The e1a3 rare variant is produced by the fusion of BCR exon 1 to ABL exon 3. The presence of this translocation has been associated with good disease outcome for chronic myeloid leukemia in a very small series of only 5 cases; there is no such evidence for B-ALL. We report two new cases of B-ALL Ph+ with the rare e1a3 fusion transcript. The e1a3 and e1a2 (p190) transcripts have been reported to have a similar molecular weight and probably a similar clinical profile, thus in these cases the presence of e1a3 was associated with extramedullary infiltration and disease acceleration.

Background

B Acute Lymphoblastic leukemia (B-ALL) with Philadelphia chromosome (Ph′) is a neoplasm of lymphoblast committed to the B-cell lineage. The clinical presentation of B-ALL Ph′+ is similar to B-ALL, but is more common in adults than in children and it has a worse prognosis among patients with B-ALL. The tyrosine kinase BCR-ABL fusion protein is the product of the Philadelphia chromosome, which results from the reciprocal translocation t(9;22)(q34;q11) that juxtaposes the c-abl oncogene 1 on chromosome 9 with the bcr gene on chromosome 22 generating the BCRABL1 oncogene [14]. Depending on the location of the breakpoint in BCR, several types of BCR-ABL fusion proteins may appear [5]. To date, three main breakpoint cluster regions in the BCR gene have been reported: the M-bcr region located between exons 12 and 16, the m-bcr located between exons e2′ and e2 and the u-bcr located in exon 19. The three more common variants of BCR-ABL described are p210 [more frequent in chronic myeloid leukemia (CML)], p190 (more frequent in B-ALL) and p230 (more frequent in Chronic Neutrophilic Leukemia (CNL) [68]. The type of rearrangement in CML is related to the patient clinical course. Other rare forms such as e1a3, b2a3 and e6a2 of BCR-ABL have been described. The e1a3 variant is produced by the fusion of BCR exon 1 to ABL exon 3. The presence of this translocation has been associated with good disease outcome for CML in a very small series of only 5 cases [9, 10]; there is no such evidence for ALL. Moreover, we have reported the first e1a3 case that progressed from CML to a lymphoid blast crisis [1]. Thus, the molecular prognosis of these rare variant forms is still unclear. We report two new cases of B-ALL Ph+ with the rare e1a3 fusion transcript.

Case 1 presentation

The first patient is a 43-year-old male, who consulted our center for night sweats and weight loss in the last 3 weeks. He had no previous history or records in our center. The physical examination was normal except for a slight splenomegaly and the blood test was compatible with hyperleukocytosis, anemia and thrombocytopenia, (leukocytes 136.00 · 109/L, hemoglobin 11.70 g/dl, platelet count 39.70 · 109/L) and 93 % of small-medium sized lymphoblast in the Grunwald-Giemsa blood smear. The bone marrow aspirate examination had an hypercellular infiltration of 90 % which was confirmed by immunophenotype (CD19+, CD10+, CD34+, TdT+, DR+, CD79 alfa+, CD20−, cytoplasm IgM negative). 80 % of the blast cells also expressed CD33+. A fresh sample from the bone marrow aspirate was collected for molecular and cytogenetic analysis. The assay revealed the presence of the following karyotype:46,XY,t(9;22)(q34;q11) [2] /46,XY,del(9)(p22)t(9;22)(q34;q11) [4] /46,XY,del(9)(p22)t(9;22)(q34;q11),del(20)(q13) [5] /46,XY [9]. FISH analysis confirmed the presence of BCR-ABL1 fusion gene (Fig. 1). The patient’s RNA was isolated from the peripheral blood and subjected to a two round multiplex reverse transcription and polymerase chain reaction (RT-PCR). In order to avoid RNA quality and/or handling errors, we included an internal positive control in where a 690-bp segment of the ubiquitously expressed transcription factor E2A mRNA was amplified. The primers and PCR conditions used in the first and second round of the nested PCR reaction are described by Pallisgaard et al. [11]. We identified an atypical amplification band of approximately 100 bp, much smaller than the p190 (e1a2) fragment. In order to confirm the presence of a BCR-ABL transcript this band was extracted from the agarose gel, purified and then analyzed by DNA sequencing. cDNA sequence revealed the presence of the e1a3 variant (Fig. 2).
Fig. 1

Cytogenetic, FISH and Immunophenotype results 1a G-banded karyotype from the sideline 46,XY,del(9)(p22)t(9;22)(q34;q11),del(20)(q13). 1b Metaphase FISH image showing BCR-ABL1 fusion gene at minor breakpoint BCR. Green BCR, red ABL1. 1c G-banded karyotype showing t(9;22)(q34;q11) as a sole chromosome alteration. 1d Metaphase FISH image showing BCR-ABL1 fusion gene at minor breakpoint BCR. LSI BCR/ABL1 dual color translocation probe (Abbott) was used. Green: BCR, Red: ABL1. 2a Immunophenotype of ALL with atypical e1a3 translocation. Presence of 90 % of blasts with lymphoblastic phenotype B: CD19+, CD10+, CD34+, DR+, CD20+, CD22−, CD33+, cytoplasmic IgM negative and positive alpha CD79. 2b Immunophenotype of ALL with atypical e1a3 translocation. Presence of 80 % of blasts with lymphoblastic phenotype B: CD19+, CD10+, CD34+, DR+, CD20+, CD22−, cytoplasmic IgM negative, positive alpha CD79 and dim expression of CD33

Fig. 2

DNA sequencing of BCR-ABL e1a3 atypical amplification band purified from both patients

The patient started induction treatment with ALL-type polychemotherapy and Imatinib, achieving complete response by morphology and immunophenotype. However, the BCR/ABL was still detected by RT-PCR at the end of the induction phase. He is ongoing consolidation with allogenic stem cell transplantation (SCT) from an HLA-identical sibling donor, 7 months after diagnosis.

Case 2 presentation

The second patient is a 65-year-old male who consulted for pain in the right leg and a history of recurrent periodontal infections in the last month. An eco-doppler confirmed a thrombophlebitis and the blood test found hyperleukocytosis and anemia (leukocytes 223.00 · 109/L, Hemoglobin 11.60 g/dl, platelet count 118 · 109/L) and the presence of 80 % lymphoblasts in the Grunwald-Giemsa blood smear. The bone marrow aspirate found an infiltration of 25 % lymphoblast which was confirmed by immunophenotype (CD19+, CD34+, DR+, CD10+, CD22+, low intensity CD20−, TdT+, cytoplasmatic IgM negative, CD33+). The karyotype was 46,XY,t(9;22)(q34;q11) [20]. FISH analysis confirmed the presence of BCR-ABL1 fusion gene (Fig. 1). The patient’s RNA was isolated from the peripheral blood. We identified an atypical e1a3 variant after a two round PCR amplification by DNA sequencing (Fig. 2).

The patient started induction treatment and Imatinib. The bone marrow aspirate on day 34 confirmed progressive disease with 30 % blast and persistence of BCR/ABL. After a second line of intensive ALL-type induction therapy the patient finally died due to toxicity (gastrointestinal bleeding). No SCT had been planned due to age and unfit status. It is noteworthy that the blood counts in a regular check-up 4 months before the diagnosis had a slight leukocytosis that was not studied.

Discussion

Overall only a few cases with the e1a3 BCRABL fusion proteins have been reported. This rare variant has mostly been described with an indolent clinical course in the setting of CML and its role in B-ALL is still unclear. We report two new cases of e1a3 Ph+ ALL patients in where we highlight the finding of the myeloid CD33+ marker expression by immunophenotype (biphenotypic and bilineal blast population were discarded by EGIL classification). This means that both cases could be defined as acute lymphoblastic leukemia with aberrant myeloid markers (ALL My+). We previously speculated that B-ALL e1a3 cases could represent CML in lymphoid blast crisis that underwent an under diagnosed chronic phase. In one of our cases we do not have any blood counts prior to the B-ALL diagnosis. However the other presented a non-evaluated leukocytosis in the blood count available before diagnosis.

A review of the bibliography of reported cases of B-ALL with the e1a3 fusion protein, using the terms “B-Acute lymphoblastic leukemia” and “e1a3” found a total of 18 cases plus the 2 being reported in this manuscript. The rare e1a3 translocation was also previously reported in the literature in 6 patients with CML in chronic phase. Furthermore, we described for the first time a patient with CML diagnosis that after 5 months of Imatinib treatment evolved to lymphoid blast crisis [1]. Table 1 shows all ALL cases with the e1a3 fusion protein previously reported as well as our 2 additional patients. The information provided by these reports shows that these cases were aggressive with 44 % of cases being refractory or relapsing after therapy and 55 % having already died at last follow-up.
Table 1

Clinical characteristics, therapy and outcome of ALL cases reported with e1a3 fusion protein compared with those presented in this manuscript

#

Age

Leukocite count at Dx

BM infiltration

TKI

SCT

Status

Response

References

1

65 years

223 × 103

25 %

Imatinib

Dead

Refractory

Current

2

43 years

133 × 103

90 %

Imatinib

AlloSCT (ongoing)

Alive

CR

Current

3

76 years

7000

85 %

Dasatinib

Alive

CR

Sonu [12]

4

Unk

5.530 × 109/L

90 %

Imatinib

Allo SCT

Alive

CR

Shin 13]

5

Unk

24.36 × 109/L

80 %

Imatinib

Dead

Not achieved

Shin [13]

6

80 years

 

94 % at blast crisis

Imatinib

Dead

CML transformed to B-ALL

Martinez-Serra [1]

7

62 years

NA

NA

Imatinib

Dead

relapse

Langabeer [14]

8

25 years

NA

NA

2d AlloSCT

Dead

Relapse/VOD

Fujisawa [15]

9

62 years

10

NA

Autologous

Dead

relapse

Burmeister [16]

10

64 years

1.8

NA

Dead

relapse

Burmeister [16]

11

31 years

31

NA

Imatinib

Allo SCT

Alive

CCR

Burmeister [16]

12

61years

14

NA

Imatinib

Alive

CCR

Burmeister [16]

13

48 years

62

NA

Dead

Relapse

Burmeister [16]

14

37 years

70

NA

Imatinib

AlloSCT

Alive

CCR

Burmeister [16]

15

45 years

5

NA

Imatinib

AlloSCT

Alive

CCR

Burmeister [16]

16

29 years

40

NA

Imatinib

AlloSCT

Alive

CCR

Burmeister [16]

17

NA

NA

NA

NA

NA

NA

NA

Wilson [17]

18

1 year

NA

NA

NA

NA

Dead

NA

Iwata [18]

19

39 years

NA

NA

NA

NA

Dead

NA

Soakerman [19]

20

NA

NA

NA

NA

NA

NA

NA

Chen [20]

B-ALL B-lymphoblastic leukemia/lymphoma, CML chronic myeloid leukemia, Allo SCT allogeneic hematopoietic stem cell transplantation, CCR clinical complete remission, TKI tyrosine kinase inhibitor, NA not available

The review of our cases and the other previously reported shows an aggressive disease similar to other Ph+ ALL. More data is needed in order to clearly define the real prognosis of B-ALL cases with e1a3 translocation and state if these patients could benefit from TKI or other approaches such as Allo SCT at consolidation. The e1a3 and e1a2 (p190) transcripts have been reported to have a similar molecular weight and probably a similar clinical profile, meaning that e1a3 could be associated with the presence of extramedullary infiltration and disease acceleration, with both patients (one achieving CR and one with PD) having persistence of e1a3 at the molecular level after induction treatment. Moreover, it is important that our reference laboratory is able to detect the rare e1a3 BCR-ABL avoiding false e1a3 negatives, in both ALL and CML.

Conclusion

We report 2 new cases of B-ALL with the rare e1a3 BCR-ABL fusion protein. At the moment the particular prognostic significance of e1a3 remains undetermined, but considering our cases and the other previously described, it seems that a clinical management with protocols of Ph+ ALL should be offered to eligible patients. The study of the clinical significance of these variants at diagnosis and follow up, is important to define if these patients have a higher risk of relapse or progressive disease in comparison to other BCR/ABL transcripts and could benefit from SCT.

Notes

Abbreviations

B-ALL: 

B Acute Lymphoblastic Leukemia B-ALL

Ph′: 

Philadelphia chromosome

CML: 

chronic myeloid leukemia

CNL: 

chronic neutrophilic leukemia

RT-PCR: 

reverse transcription and polymerase chain reaction

FISH: 

fluorescence in situ hybridization

Allo SCT: 

allogeneic hematopoietic stem cell transplantation

TKI: 

tyrosine kinase inhibitor

CR: 

complete remission

PD: 

progressive disease

Declarations

Authors’ contributions

JCA, JM-S, LG, MB, RR, JLL, MS and FS carried out the molecular genetic studies, participated in the sequence alignment and drafted the manuscript. JI and VC carried out the immunoassays. JM-S, AS, AG, MAD participated in the design of the study and performed the statistical analysis. BLA, FS and JM-S conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Acknowledgements

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Consent

Written informed consent was obtained from the patient’s next of kin for publication of this case report and any accompanying images.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Department of Hematology, University Hospital Son Espases
(2)
Department of Genetics, University Hospital Son Espases
(3)
Department of Immunology, University Hospital Son Espases
(4)
Department of Pathology, University Hospital Son Espases
(5)
Instituto de Investigación Sanitaria de Palma (IdISPa)

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Copyright

© López-Andrade et al 2016

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