Fig. 2

Histopathological analysis of pre-treatment tumor tissue. Pre-treatment formalin-fixed paraffin-embedded tissue specimen was stained for CD3, CD8 and PD-L1 by immunohistochemistry. Sections indicate presence of CD3+ and CD8+ T-cells though CD3+ T-cells outnumbered CD8+ T-cells. PD-L1 staining was heterogenous, localized to tumor tissue and of moderate intensity (2+, using method previously described by Taube JM et al., Sci Transl Med 2012)