A case of minor BCR-ABL1 positive acute lymphoblastic leukemia following essential thrombocythemia and originating from a clone distinct from that harboring the JAK2-V617F mutation
Experimental Hematology & Oncology volume 3, Article number: 6 (2014)
Here we report on a case of Philadelphia chromosome positive B lymphoblastic leukemia (Ph+ALL), which developed following a long duration of essential thrombocythemia (ET). A mutational analysis of Janus Kinase 2 (JAK2) revealed that the V617F mutation was present in granulocytes and in hematopoietic stem and progenitor cells (HSPCs), but not in the CD34+CD19+ population that mostly consists of Ph+ALL cells, indicating that this Ph+ALL clone did not originate from the ET clone carrying the JAK2-V617F mutation. The minor BCR-ABL1 fusion was detected not only in the CD34+CD19+ population but also in HSPCs and granulocytes, indicating that the Philadelphia chromosome was acquired in an early hematopoietic stage at least prior to the commitment to B cell development. Upon dasatinib treatment, the minor BCR-ABL1 transcript rapidly disappeared in HSPCs but persisted in the CD34+CD19+ population. A relapse of Ph+ALL occurred nine months later without the disappearance of the minor BCR-ABL1 transcript in the bone marrow cells during the treatment course, suggesting that a resistant Ph+ALL clone may have arisen or been selected in the committed B cells rather than in HSPCs. This case report may partly contribute to filling the gap between previous data acquired from mice experiments and the phenomenon in real patients.
Myeloproliferative neoplasms (MPNs) are a group of stem cell disorders including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), all of which are characterized by the overproduction of mature blood cells. It is well known that patients with MPNs often develop acute myeloid leukemia (AML) , but also it has been occasionally reported that MPNs may be associated with lymphoid malignancies including non-Hodgkin lymphoma, chronic lymphocytic leukemia (CLL), and multiple myeloma [2, 3]. However, a genetic association between B lymphoblastic leukemia (B-ALL) and MPNs is rarely observed [4, 5].
The JAK2-V617F mutation is one of the major causes of MPNs and is present in the vast majority of these patients (90–95% of PV patients and 50–60% of ET and PMF patients) . Intriguingly, transformation of the JAK2-V617F positive clones to AML is observed mainly in cases of primary or secondary myelofibrosis while AML clones arising directly from PV and ET are mostly JAK2 wild-type, indicating clonal heterogeneity of MPNs . Similarly, in cases of CLL or diffuse large B cell lymphoma following MPNs, the JAK2- V617F mutation is detected either in both MPN cells and B lymphoid tumor cells or in only MPN cells . JAK2 mutations at other residues, such as R683, are also observed in high-risk childhood acute lymphoblastic leukemia , supporting the theory that JAK2 mutations may confer a growth advantage on B lymphocytes.
The BCR-ABL1 fusion kinase encoded by the Philadelphia (Ph) chromosome, which arises from the chromosomal translocation t(9;22), is a major cause of chronic myeloid leukemia (CML) as well as of Ph+ acute lymphoblastic leukemia (Ph+ALL). CML is presumed to arise from aberrant Ph+ stem cells which are enriched in the CD34+CD38- hematopoietic stem cell population. Tyrosine kinase inhibitors (TKIs) such as imatinib and dasatinib that specifically target the BCR-ABL1 kinase have improved the outcomes of patients with CML but have failed to provide a cure for the disease. The maintenance of leukemia stem cells (LSCs), which are capable of engraftment in immunodeficient mice, does not require the BCR-ABL1 kinase activity , and hence the disease usually relapses once TKI treatment is discontinued .
Ph+ALL is a subtype of B-ALL with a particularly poor prognosis even in the current era of TKIs. It remains unclear whether Ph+ALL arises from the CD34+CD19- population before the commitment to B cell development  or from the CD34+CD19+ pro-B population . LSCs of B-ALL do not appear to be enriched in a specific population in xenotransplanted mice [12, 13] but it is unclear whether a specific population resistant to TKIs, as in the case of CML-LSCs, exists in Ph+ALL patients.
In this case report, we describe a particular case of Ph+ALL that followed ET, examine which cell population is the target for two major genetic alterations, JAK2-V617F and minor BCR-ABL1 to understand the clonal architecture between Ph+ALL and ET, and investigate whether the sensitivity of subpopulations of Ph+ALL to dasatinib differs.
In 1995, a 51-year-old woman was diagnosed as ET with the clinical examinations revealing a platelet count of 1240 × 109 /L, total white blood cell count of 7.9 × 109 /L, hemoglobin levels of 12.1 g/dl, and a marked proliferation of large, mature megakaryocytes in the bone marrow aspirate. She had been treated with only an anti-thrombotic agent for more than ten years except for one year with a cytoreductive therapy utilizing hydroxyurea that was discontinued in 2003 due to intolerance, and her disease had been well controlled without any thrombotic events or any signs of progression to terminal myelofibrosis. In October 2011, at the age of 67, the platelet count suddenly decreased to 336 × 109 /L and blasts were detected with a total leukocyte count of 8.9 × 109 /L (14% blasts) in the peripheral blood. Computed tomography (CT) scans of the abdomen and pelvis showed no splenomegaly. A bone marrow examination revealed hypercellularity with increased numbers of megakaryocytes and leukemic blasts, accounting for 76% of the total nucleated cells. Fluorescence-activated cell sorting (FACS) analysis showed a B-ALL phenotype (CD34+ CD19+ CD10+ CD13+ HLA-DR+) and Southern blot analysis clearly demonstrated monoclonality with a rearrangement of the immunoglobulin heavy chain gene. Cytogenetic analysis as well as Fluorescence in situ hybridization (FISH) analysis revealed clonal abnormalities with translocation t(9;22)(q34; q11.2) of the Ph chromosome and monosomy 7. The presence of the minor BCR-ABL1 fusion transcript was confirmed using a reverse transcription–polymerase chain reaction (RT-PCR) and direct Sanger sequencing. Based on these results, we diagnosed Ph+ALL that may have transformed from ET.
Subsequently, after acquiring a written informed consent, we investigated the JAK2-V617F mutational status in peripheral granulocytes isolated by Percoll density gradient centrifugation and in FACS-sorted lineage-CD34+ HSPCs and CD34+CD19+ B-ALL cells from peripheral blood mononuclear cells (PBMCs) at diagnosis. The sequencing analysis performed as previously described  showed that the JAK2-V617F mutation was present clearly in granulocytes and to a lesser extent in HSPCs, but not at all in B-ALL cells (Figure 1A). These results indicate that the B-ALL clone did not originate from the ET clone with the JAK2-V617F mutation.
Next in order to determine the stage in which the Ph chromosome was initially acquired, we separated CD34+ cells into four populations according to CD10 and CD19 expression (Figure 1B) after the exclusion of populations with lineage markers other than B cell markers including CD10, CD19, and CD20, and then performed the RT-PCR for the minor BCR-ABL1 transcript. As expected, the amplification of the transcript was observed in the CD34+CD19+CD10+ and the CD34+CD19+CD10- populations both of which are committed to B cell development. However, the CD34+CD19-CD10- population which enriches HSPCs also expressed the minor BCR-ABL1 transcript (Figure 1C). FISH analysis revealed that 54% of CD34+CD19-CD10- cells as well as 10% of granulocytes that are defined as segmented nuclear cells carried the Ph chromosome (Figure 1D). Taken together, these findings suggest that the Ph chromosome was acquired during an early hematopoietic stage before the commitment to B cell development.
After the diagnosis, the patient was treated with dasatinib and prednisolone. Four weeks later, we observed a great reduction of leukemia cells. To investigate whether the most primitive population in Ph+ALL were less sensitive to TKIs as in the case of CML, we separated bone marrow mononuclear cells (BMMCs) into three populations according to the expression level of CD34 and CD19 (Figure 2A) and performed RT-PCR for minor BCR-ABL1. Contrary to our expectation, the minor BCR-ABL1 transcript was no longer detected in the CD34+CD19- population that was almost negative for CD10 corresponding to the CD34+CD19-CD10- population depicted in Figure 1B-D, which carried the minor BCR-ABL1 transcript at diagnosis. However, the transcript was still detected in the CD34+CD19+ population (Figure 2B). Ten weeks later, the patient achieved cytogenetic remission with an increase in the platelet count, suggesting that the ET clone had repopulated during treatment. However, the minor BCR-ABL1 transcript was still detected at low levels in the bulk bone marrow cells (Figure 2B), and finally the Ph+ALL relapsed with the T315I mutation nine months later, indicating that a resistant clone may not always derive from the most primitive population such as HSPCs.
Cases of ET and B-ALL comorbidity are very rare. We initially thought this case was a transformation of ET to B-ALL similar to lymphoid crisis of CML, but were proven wrong when the mutational analysis of JAK2 clearly showed that the B-ALL clone did not originate from the ET clone with the JAK2-V617F mutation. These results raise two hypotheses. One is that a microenvironment generated by MPNs may contribute to the development of an aberrant clone. A recent report that MPNs can remodel the bone marrow niche may support this hypothesis . The other is that an aberrant clone may develop independently to ET or B-ALL with the additional hit of the JAK2-V617F mutation or the translocation t(9;22) respectively. A previous report that del (11q) was detected both in a JAK2-V617F positive MPN clone and in a JAK2-V617F negative AML clone in the same patient may support this hypothesis . However, Monosomy 7 which was positive at diagnosis of Ph+ALL was not detected in bone marrow cells in cytogenetic remission after dasatinib treatment, indicating it was probably a second hit after the translocation t(9;22) in Ph+ALL cells. Since mutations in epigenetic regulators are common in MPNs , we also performed the mutational analysis for several genes such as the terminal exon of DNMT3A including R882, exon 4 of IDH1 including R132, exon 4 of IDH2 including R140 and R172, and exon 3 to 11 of TET2, but failed to find any founder mutations. The recent progress of high-throughput sequencing may resolve this question in the future.
LSCs of CML are enriched in the CD34+CD38- hematopoietic stem cell population while those of B-ALL are not enriched in a specific population as several reports demonstrated that various phenotypically separated populations such as HSPCs and pre-B cells possess the engraftment capacity in immunodeficient mice [12, 13]. Given that LSCs of CML are resistant to TKIs due to their quiescence and independent maintenance from BCR-ABL kinase , LSCs of B-ALL might be defined as a population that is not eliminated by chemotherapy and TKI treatment in patients. However, they have not yet been well studied in real Ph+ALL patients treated with TKIs. The LSC-like population of Ph+ALL in this case might exist in the CD34+CD19+ B cell committed population rather than in lineage-CD34+ HSPCs, since the BCR-ABL1 transcript remained in the former but quickly disappeared in the latter at four weeks after therapy initiation, despite the latter being more primitive. Although we could not analyze whether the T315I mutation was acquired or selected in this committed population due to the limited sample size, it would be of value to examine similar cases in more detail throughout the clinical courses, in order to fill the current knowledge gap between results from mice experiments and findings from real patients and to be able to eradicate residual leukemic clones in such patients.
Written informed consent was obtained from the patient for publication of this Case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
Janus Kinase 2
Hematopoietic stem and progenitor cells
Acute myeloid leukemia
Chronic lymphocytic leukemia
Chronic myeloid leukemia
Tyrosine kinase inhibitors
Leukemia stem cells
Fluorescence-activated cell sorting
Fluorescence in situ hybridization
Reverse transcription–polymerase chain reaction
Peripheral blood mononuclear cells
Bone marrow mononuclear cells.
Beer PA, Delhommeau F, LeCouedic JP, Dawson MA, Chen E, Bareford D, Kusec R, McMullin MF, Harrison CN, Vannucchi AM, Vainchenker W, Green AR: Two routes to leukemic transformation after a JAK2 mutation-positive myeloproliferative neoplasm. Blood 2010, 115: 2891–2900. 10.1182/blood-2009-08-236596
Musolino C, Allegra A, Penna G, Centorrino R, Cuzzola M, D’Angelo A, Iacopino P, Alonci A: Absence of the V617F JAK2 mutation in the lymphoid compartment in a patient with essential thrombocythemia and B-chronic lymphocytic leukemia and in two relatives with lymphoproliferative disorders. Acta Haematol 2009, 122: 46–49. 10.1159/000243721
Vannucchi AM, Masala G, Antonioli E, Chiara Susini M, Guglielmelli P, Pieri L, Maggi L, Caini S, Palli D, Bogani C, Ponziani V, Pancrazzi A, Annunziato F, Bosi A: Increased risk of lymphoid neoplasms in patients with Philadelphia chromosome-negative myeloproliferative neoplasms. Cancer Epidemiol Biomarkers Prev 2009, 18: 2068–2073. 10.1158/1055-9965.EPI-09-0353
Woronzoff-Dashkoff KK, Litz CE: Acute lymphoblastic leukemia in a case of essential thrombocythemia. Am J Clin Pathol 1996, 106: 206–208.
Jurisic V, Colovic N, Terzic T, Djordjevic V, Colovic M: Transformation of primary myelofibrosis with 20q- in Philadelphia-positive acute lymphoblastic leukemia: case report and review of literature. Pathol Res Pract 2012, 208: 420–423. 10.1016/j.prp.2012.04.007
Levine RL: JAK-mutant myeloproliferative neoplasms. Curr Top Microbiol Immunol 2012, 355: 119–133.
Bercovich D, Ganmore I, Scott LM, Wainreb G, Birger Y, Elimelech A, Shochat C, Cazzaniga G, Biondi A, Basso G, Cario G, Schrappe M, Stanulla M, Strehl S, Haas OA, Mann G, Binder V, Borkhardt A, Kempski H, Trka J, Bielorei B, Avigad S, Stark B, Smith O, Dastugue N, Bourquin JP, Tal NB, Green AR, Izraeli S: Mutations of JAK2 in acute lymphoblastic leukaemias associated with Down’s syndrome. Lancet 2008, 372: 1484–1492. 10.1016/S0140-6736(08)61341-0
Corbin AS, Agarwal A, Loriaux M, Cortes J, Deininger MW, Druker BJ: Human chronic myeloid leukemia stem cells are insensitive to imatinib despite inhibition of BCR-ABL activity. J Clin Invest 2011, 121: 396–409. 10.1172/JCI35721
Mahon FX, Rea D, Guilhot J, Guilhot F, Huguet F, Nicolini F, Legros L, Charbonnier A, Guerci A, Varet B, Etienne G, Reiffers J, Rousselot P: Discontinuation of imatinib in patients with chronic myeloid leukaemia who have maintained complete molecular remission for at least 2 years: the prospective, multicentre Stop Imatinib (STIM) trial. Lancet Oncol 2010, 11: 1029–1035. 10.1016/S1470-2045(10)70233-3
Hotfilder M, Rottgers S, Rosemann A, Schrauder A, Schrappe M, Pieters R, Jurgens H, Harbott J, Vormoor J: Leukemic stem cells in childhood high-risk ALL/t(9;22) and t(4;11) are present in primitive lymphoid-restricted CD34 + CD19- cells. Cancer Res 2005, 65: 1442–1449. 10.1158/0008-5472.CAN-04-1356
Castor A, Nilsson L, Astrand-Grundstrom I, Buitenhuis M, Ramirez C, Anderson K, Strombeck B, Garwicz S, Bekassy AN, Schmiegelow K, Lausen B, Hokland P, Lehmann S, Juliusson G, Johansson B, Jacobsen SE: Distinct patterns of hematopoietic stem cell involvement in acute lymphoblastic leukemia. Nat Med 2005, 11: 630–637. 10.1038/nm1253
le Viseur C, Hotfilder M, Bomken S, Wilson K, Rottgers S, Schrauder A, Rosemann A, Irving J, Stam RW, Shultz LD, Harbott J, Jurgens H, Schrappe M, Pieters R, Vormoor J: In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties. Cancer Cell 2008, 14: 47–58. 10.1016/j.ccr.2008.05.015
Kong Y, Yoshida S, Saito Y, Doi T, Nagatoshi Y, Fukata M, Saito N, Yang SM, Iwamoto C, Okamura J, Liu KY, Huang XJ, Lu DP, Shultz LD, Harada M, Ishikawa F: CD34 + CD38 + CD19+ as well as CD34 + CD38-CD19+ cells are leukemia-initiating cells with self-renewal capacity in human B-precursor ALL. Leukemia 2008, 22: 1207–1213. 10.1038/leu.2008.83
Steensma DP, Dewald GW, Lasho TL, Powell HL, McClure RF, Levine RL, Gilliland DG, Tefferi A: The JAK2 V617F activating tyrosine kinase mutation is an infrequent event in both “atypical” myeloproliferative disorders and myelodysplastic syndromes. Blood 2005, 106: 1207–1209. 10.1182/blood-2005-03-1183
Schepers K, Pietras EM, Reynaud D, Flach J, Binnewies M, Garg T, Wagers AJ, Hsiao EC, Passegue E: Myeloproliferative neoplasia remodels the endosteal bone marrow niche into a self-reinforcing leukemic niche. Cell Stem Cell 2013, 13: 285–299. 10.1016/j.stem.2013.06.009
Theocharides A, Boissinot M, Girodon F, Garand R, Teo SS, Lippert E, Talmant P, Tichelli A, Hermouet S, Skoda RC: Leukemic blasts in transformed JAK2-V617F-positive myeloproliferative disorders are frequently negative for the JAK2-V617F mutation. Blood 2007, 110: 375–379. 10.1182/blood-2006-12-062125
Shih AH, Abdel-Wahab O, Patel JP, Levine RL: The role of mutations in epigenetic regulators in myeloid malignancies. Nat Rev Cancer 2012, 12: 599–612. 10.1038/nrc3343
This work was funded by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MK and MH). We are very grateful to Dr. Nobumasa Inoue (National Hospital Organization Osaka National Hospital) for supplying the clinical record of the patient.
The authors declare that they have no relevant financial interests.
YN performed all experiments and wrote the manuscript. MK designed the study and all experiments, and wrote the manuscript. KY cared for the patient. NS and YS performed a part of experiments. MH, NK and AT helped to draft the manuscript. All authors read and approved the final manuscript.
About this article
Cite this article
Nagai, Y., Kawahara, M., Sugino, N. et al. A case of minor BCR-ABL1 positive acute lymphoblastic leukemia following essential thrombocythemia and originating from a clone distinct from that harboring the JAK2-V617F mutation. Exp Hematol Oncol 3, 6 (2014). https://doi.org/10.1186/2162-3619-3-6