Roles of Polo-like kinase 3 in suppressing tumor angiogenesis
© Xu et al; licensee BioMed Central Ltd. 2012
Received: 14 February 2012
Accepted: 18 April 2012
Published: 18 April 2012
Angiogenesis is essential for promoting growth and metastasis of solid tumors by ensuring blood supply to the tumor mass. Targeting angiogenesis is therefore an attractive approach to therapeutic intervention of cancer. Tumor angiogenesis is a process that is controlled by a complex network of molecular components including sensors, signaling transducers, and effectors, leading to cellular responses under hypoxic conditions. Positioned at the center of this network are the hypoxia-inducible factors (HIFs). HIF-1 is a major transcription factor that consists of two subunits, HIF-1α and HIF-1β. It mediates transcription of a spectrum of gene targets whose products are essential for mounting hypoxic responses. HIF-1α protein level is very low in the normoxic condition but is rapidly elevated under hypoxia. This dramatic change in the cellular HIF-1α level is primarily regulated through the proteosome-mediated degradation process. In the past few years, scientific progress has clearly demonstrated that HIF-1α phosphorylation is mediated by several families of protein kinases including GSK3β and ERKs both of which play crucial roles in the regulation of HIF-1α stability. Recent research progress has identified that Polo-like kinase 3 (Plk3) phosphorylates HIF-1α at two previously unidentified serine residues and that the Plk3-mediated phosphorylation of these residues results in destabilization of HIF-1α. Plk3 has also recently been found to phosphorylate and stabilize PTEN phosphatase, a known regulator of HIF-1α and tumor angiogenesis. Given the success of targeting protein kinases and tumor angiogenesis in anti-cancer therapies, Plk3 could be a potential molecular target for the development of novel and effective therapeutic agents for cancer treatment.
When tumors grow to a certain size, a severe hypoxic microenvironment develops within the tumor mass . Simple diffusion of oxygen and nutrients becomes insufficient to meet the demand of fast growing tumors . It is believed that tumor size generally will not exceed 1 centimeter in diameter without the support of angiogenesis . Tumor angiogenesis is a process leading to formation of vasculature that supplies the growth of solid tumors [1, 2]. Logically, tumor angiogenesis tends to be more pronounced in aggressive and malignant tumors . Since the initial discovery of tumor angiogenesis four decades ago , tumor angiogenesis has emerged as an important target for cancer therapy [1, 2, 5–7]. Many inhibitors of tumor angiogenesis have been developed and used in clinical application or is in the pipeline leading to clinics [1, 2, 6, 7]. Since highly vascular tumors tend to have much higher potential for metastasis , these inhibitors of angiogenesis may have the added value of blocking tumor metastasis, thus making them more effective for cancer treatment.
A primary consequence of increased tumor size is hypoxia, a result of oxygen restriction in the tumor mass. Hypoxia triggers tumor angiogenesis by activating the cellular hypoxia response pathways . Located in the center of these pathways are the hypoxia inducible factors (HIFs) including the well characterized HIF-1 [1, 9–11]. HIF-1 is a transcription factor that promotes transcription of a series of genes such as vascular endothelial growth factor (VEGF) that are critical for the cellular hypoxic response [1, 5].
HIF-1α is the inducible subunit of the HIF-1 transcription factor, whose protein level can be dramatically induced upon exposure to hypoxia [1, 9–11]. The cellular level of HIF-1α protein is regulated primarily at the post-translational level [1, 9–11]. The HIF-1α gene is constitutively transcribed and translated [1, 9–11]. Under normoxic conditions, HIF-1α protein is hydroxylated by prolyl hydroxylases (PHDs), recognized and polyubiquitinated by and degraded by a proteosome-dependent mechanism [1, 9–11]. Hypoxia greatly stabilizes HIF-1α because of a reduced activity of PHDs due to low oxygen tension [1, 9–11]. In addition to hydroxylation, phosphorylation by protein kinases also regulates HIF-1α stability and/or localization [12–17]. Compared to hydroxylation, the significance of HIF-1α phosphorylation in regulating its stability is far less appreciated. Most recent studies have demonstrated that Plk3 is an endogenous kinase of HIF-1α that regulate its stability . In addition, Plk3 phosphorylates and stabilizes PTEN phosphatase , which can in turn contribute to HIF-1α stability by suppression of the PDK/Akt signaling axis. Supporting this, PLK3 knock-out murine embryonic fibroblasts (MEFs) display significantly enhanced expression of HIF-1α and tumor angiogenesis in response to hypoxia [17, 19]. In this review, we will discuss significance of Plk3 in HIF-1α regulation, tumor angiogenesis, and its potential as a therapeutic target for cancer treatment.
Polo-like kinase 3
Murine Plk3 was initially identified as a fibroblast growth factor-inducible kinase (Fnk) in NIH 3T3 cells in a differential display study . Human Plk3 was later cloned and named as Prk (p roliferation-r elated k inase) . The amino acid sequence of human Plk3 shares 36% and 33% overall identity to Plk1 and Polo, respectively . Plk3 has the structure that is typical of a Plk family member, with an N-terminal kinase domain and a C-terminal PBD with two Polo boxes  (Figure 1).
The Plk3 mRNA level was found to be inducible by mitogen stimulation with peak expression at the G1 phase, whereas its protein level seems to be fairly constant in mitogen-stimulated cells throughout the cell cycle [25, 36, 37]. However, a more recent report, shows that Plk3 protein level is also cell cycle-regulated with a pattern similar to that of its mRNA . Plk3 is considered an immediate early response gene, whose expression does not depend on de novo protein synthesis [25, 33, 35]. The kinase activity of Plk3 has also been shown to oscillate during the cell cycle and it is subjected to regulation by a variety of stress conditions, including genotoxic insults, hypoxic treatment, and osmotic shock [27–29, 36, 39–41].
The function of Plk3 appears to be rather diverse. Plk3 is involved in multiple phases of the cell cycle [38, 42]. Plk3 is closely associated with spindle poles, mitotic spindles, and midbody during mitosis . Plk3 overexpression induces cell cycle arrest at the M phase followed by apoptosis, which likely results from deregulated microtubule dynamics and centrosomal function . Plk3 may also regulate the onset and/or progression of mitosis and meiosis . Plk3 phosphorylates and regulates the subcellular localization of Cdc25C phosphatase [44, 45], a key regulator of the G2/M transition . Plk3 is reported to mediate the G1/S transition and appears to be required for the S phase entry [38, 47]. In addition, Plk3 phosphorylates proteins that are important for regulating DNA replication, such as topoisomerase IIα and DNA polymerase δ (Pol δ) [42, 48, 49]. Furthermore, Plk3 regulates Golgi fragmentation during mitotic entry in mammalian cells [50–52].
Plk3 and tumor angiogenesis
Plk3 regulates tumor angiogenesis through HIF-1α
Neovascularization as the result of angiogenesis is essential for progression of solid tumor as the fast growing tumor mass needs to be supported by oxygen and nutrients from the blood [1, 2]. One of the key events that triggers tumor angiogenesis is cellular hypoxia, which is a consequence of blood restriction in the inner portion of a solid tumor [2, 3, 8]. Thus, cellular responses to hypoxia play a pivotal role in tumor angiogenesis [2, 3, 8].
HIF-1 is the primary player in cellular hypoxic responses and the initiating factor for tumor angiogenesis [1, 9, 10]. HIF-1 is an oxygen sensing transcription factor containing a constitutively expression β subunit (HIF-1 β) and an inducible α subunit (HIF-1α) [1, 10, 11, 62]. Upon activation by hypoxia, HIF-1 trans-activates an array of genes whose products in turn drive the cellular machinery for hypoxic responses [1, 10, 11]. Among the most important HIF-1 inducible proteins are VEGF, fibroblast growth factor (FGF), and glucose transporter [1, 2]. These proteins are known to stimulate the formation of vasculature or nutrient transport [1, 2].
The association of Plk3 with tumor angiogenesis was discovered in a recent genetic study demonstrating that PLK3 null mice display an increased tumor incidence . Importantly, tumors developed in these mice are frequently large in mass and more vasculated . Further biochemical analysis revealed that Plk3 modulates HIF-1α protein levels in response to hypoxic conditions [17, 19]. Specifically, compared with wild-type mouse embryonic fibroblasts (MEFs), PLK3 null MEFs exhibit an elevated level of HIF-1α and a hyper-sensitive response to the treatment with hypoxia or with nickel ions that elicit hypoxia-like cellular responses [17, 19]. Consistently, expression of VEGF-A, a major HIF-1α response protein, is also higher in PLK3 -/- MEFs than in wild-type MEFs . Ex vivo analyses confirmed the role of Plk3 in regulating the stability of HIF-1α [17, 19]. Furthermore, ectopically expressed Plk3 was able to suppress the nuclear accumulation of HIF-1α in HeLa cells . This inhibition of HIF-1α nuclear translocation appears to be dependent on the kinase activity of Plk3 since overexpression of only the Plk3 kinase domain is sufficient to suppress HIF-1α accumulation in the nucleus under hypoxic conditions . These studies strongly suggest that Plk3 regulates tumor angiogenesis through modulating the expression level of HIF-1α.
Plk3 directly phosphorylates HIF-1α and regulates its stability
Phosphorylation of HIF-1α by several protein kinases has been reported to regulate HIF-1α stability and/or nuclear translocation [12–17, 19]. The MAP kinases, ERKs, phosphorylate HIF-1α at residues Ser-641 and Ser-643  (Figure 5). This phosphorylation facilitates translocation of HIF-1α from the cytoplasm to the nucleus, thereby promoting HIF-1-mediated transcriptional activity [15, 16]. These phosphorylation sites are located far outside of the nuclear localization signal (NLS) but within the nuclear exporting signal (NES) of HIF-1α, suggesting that phosphorylation of these sites regulates nuclear export rather than import of HIF-1α  (Figure 5). Glycogen synthase kinase 3 β (GSK3β) phosphorylates HIF-1α at three serine residues (Ser-551, Ser-555, and Ser-589) located within its oxygen-dependent degradation domain (ODDD)  (Figure 5). Phosphorylation at these residues by GSK3β enhances HIF-1α degradation in a pVHL-independent manner, resulting in suppression of the HIF-1 activity [12, 13].
A recent study identifies Plk3 as a new player in the regulation of HIF-1α stability through direct phosphorylation . This study was prompted by genetic evidence showing the involvement of Plk3 in tumor angiogenesis and HIF-1α regulation . The most obvious possibility is that Plk3 phosphorylates HIF-1α and destabilizes HIF-1α in a manner similar to that of GSK3β [12, 13]. Kinase assays using purified recombinant HIF-1α and Plk3 reveal that Plk3, but not the kinase-dead counterpart, strongly phosphorylates HIF-1α in vitro . HIF-1α phosphorylation by Plk3 results in a significant mobility shift on a denaturing gel . Pull-down and Co-IP analyses confirm that Plk3 interacts with HIF-1α . Two serine residues (Ser-576 and Ser-657) of HIF-1α have subsequently been identified as direct targets of Plk3 by mass spectrometry  (Figure 5). These two residues are evolutionarily conserved among higher animals and have not been shown to be targets of any other kinases . Ser-576 is located within ODDD whereas Ser-657 is right downstream of the NES of HIF-1α (Figure 5), suggesting that Plk3 can potentially regulate both HIF-1α degradation and its nuclear localization. Further analyses reveal that recombinant HIF-1α proteins containing mutations of these residues were much more stable when they are ectopically expressed in cells . Specifically, a single mutation that replaces either Ser-576 or Ser-657 with alanine greatly stabilizes the HIF-1α protein level when the mutant is expressed in HEK293 cells . Replacing both residues with alanines further stabilizes HIF-1α , underscoring the importance of phosphorylation of these residues in controlling HIF-1α stability. It is worthwhile to note that mutation of Ser-576, which is located within the ODDD of HIF-1α, appears to be more effective in stabilizing HIF-1α than mutation of Ser-657. These results are consistent with the previous finding that phosphorylation of residues in this domain by GSK3β compromises HIF-1α stability . Similar to that of GSK3β, the Plk3-dependent destabilization of HIF-1α also appears to be hydroxylation- and pVHL-independent as mutations of Plk3-phosphorylation sites can further stabilize HIF-1α with both key proline residues for hydroxylation mutated to alanines [12, 17].
Plk3 indirectly regulates HIF-1a through PTEN phosphatase
PTEN and tumor angiogenesis
The lipid phosphatase and tensin homologue (PTEN) is an important tumor suppressor that is mutated or deleted in many cancers [63, 64]. PTEN inhibits the phosphatidylinositol 3-kinases kinase (PI3K) signaling pathway by dephosphorylating the phosphoinositides on the 3' position of the inositol ring, thereby reversing the effect of PI3K leading to activation AKT [65, 66]. The close tie between PTEN and tumor angiogenesis has been well established [67–71]. PTEN is capable of suppressing tumor angiogenesis in vivo in multiple tumors [67–71]. The inhibitory effect of PTEN on tumor angiogenesis is through the inhibition of the PI3K signaling cascade leading to activation of AKT [67–71]. The PI3K pathway is known to facilitate tumor angiogenesis by increasing the HIF-1α level [5, 11, 68]. Accordingly, growth factors and cytokines, such as EGF and insulin, increase HIF-1α protein level , since these factors activate the PI3K pathway . AKT may increase HIF-1α through its downstream targets such as mTOR or GSK3β, which regulate the protein synthesis and stability of HIF-1α, respectively [5, 11, 12]. A more recent study provided direct evidence that GSK3β directly phosphorylates HIF-1α and promotes its degradation in a hydroxylation-independent manner  (see previous section). In the PI3K signaling cascade, AKT directly phosphorylates GSK3β at Ser-9, thereby inhibiting its activity [72–74]. Thus, PTEN inhibits tumor angiogenesis by suppressing HIF-1 activity through the reduction of the HIF-1α protein level. This is accomplished by the inhibition of the PI3K pathway that leads to lower GSK3β activity.
Regulation of PTEN by phosphorylation
The effect of PTEN phosphorylation on PTEN function appears to be multifaceted. Phosphorylation of Ser-370 and Ser-385 by CK2 has been reported to suppress the PTEN activity [76, 77]. So does the tyrosine phosphorylation by Src . In contrast, mutations of Ser-380, Ser-382, and Ser-383 destabilize PTEN, suggesting that phosphorylation of these sites may increase its stability [85, 88]. Phosphorylation of PTEN by CK2 at Ser-370 and Ser-385 and by Plk3 at Thr 366 and Ser-370 also stabilize PTEN [18, 77]. Interestingly, deletion of the tail-domain or mutation of Ser-380, Ser-382, and Ser-383 greatly reduces the stability PTEN but activates its phosphatase activity [84, 88]. A model has been proposed that upon phosphorylation, the C-terminal domain, stabilizes the protein due to a change in protein conformation that inhibits its phosphatase activity [84, 87, 88]. Dephoshorylation of PTEN releases the inhibitory conformation and renders PTEN more active [84, 87, 88]. However, a change in the overall PTEN level can have great biological consequences . For instance, haploinsufficiency of PTEN also results in enhanced tumor development [90, 91], underscoring the importance of the PTEN protein level in controlling cell proliferation and malignant transformation. Given the fact that PTEN is phosphorylated by multiple protein kinases, it is conceivable that a correct combination of phosphorylation is needed to fine-tune the activity or level of PTEN for a given physiological state.
Plk3 phosphorylates and regulates PTEN stability
PLK3 ablation also results in significantly increased activity of the PI3K signaling pathway. Specifically, enhanced phosphorylation of Ser-473 of AKT, Ser-9 of GSK3β, and Ser-2448 of mTOR were observed in PLK3 null mouse embryonic fibroblasts (MEFs) compared to wild-type MEFs . Further analyses on the upstream components of the PI3K pathway revealed that PLK3 null MEFS contained lower levels of PTEN protein . These findings raised the possibility that Plk3 may directly regulate PTEN by phosphorylation. In vitro kinase assays using recombinant PTEN and Plk3 confirmed that Plk3 indeed strongly phosphorylates PTEN . Mass spectrometric analysis identified Thr-366 and Ser-370 as residues that were phosphorylated by Plk3 . The phosphorylation of these two sites by Plk3 was further confirmed using a phospho-specific antibody that recognized phospo-Thr-366 and phospo-Ser-370 .
Phosphorylation of these two sites was found to affect the stability of PTEN. PTEN constructs with Thr-366 and Ser-370 mutated to alanines were less stable when transfected into HEK 293 cells . This result is therefore consistent with the reduced endogenous PTEN protein level in PLK3 null MEFs . The lower PTEN level in PLK3 null MEFs corresponds to a reduced overall PTEN activity as phosphorylation of phosphoinositide-dependent protein kinase 1 (PDK-1) at Ser-241, an indirect target of PTEN , was enhanced in PLK3 null MEFs . Thus, phosphorylation of Thr-366 and Ser-370 by Plk3 functions to stabilize PTEN, resulting in an increased overall PTEN phosphatase activity in the cell. Of note, these two sites have been reported to be the targets of CK2 [76, 77]. Thr-366 can also be phosphorylated by GSK3β . Therefore, Thr-366 and Ser-370 of PTEN appear to be subjected to modification by multiple protein kinases.
PTEN stability affected by Plk3 phosphorylation appears to be proteosome-dependent since proteosome inhibitor was able to increase PTEN protein level in PLK3 null MEFs . This is consistent with previous findings that PTEN is subjected to ubiquitination and degradation by the proteosome [85, 93, 94]. Notably, there is considerable phosphorylation of Ser-370 and Thr-366 of PTEN at the resting state that is readily detectable by the phospho-specific antibody . This constitutive phosphorylation is partially inhibited by LiCl, an inhibitor of GSK3β and CK2 , supporting the previous findings that one or both of these two kinases phosphorylate these two sites [76, 77].
Plk3 as a potential therapeutic target for cancer treatment
The newly discovered role of Plk3 in tumor angiogenesis highlights its potential as a therapeutic target for cancer treatment. Tumor angiogenesis has been well established as a valuable cellular process for drug intervention [2, 6, 7]. Protein kinases have been favored by the pharmaceutical industry for cancer drug development . Screening small chemicals that interfere with the ATP binding capacity of kinases thereby inhibiting their kinase activities is the conventional approach for identifying drug leads [96, 97]. Among the Plk family kinases, Plk1 has been the major focus of drug development [58, 98, 99]. Several ATP-competitive and non-ATP competitive compounds have been identified as having the potential to inhibit Plk1 activity [58, 98, 99]. These compounds possess the ability, albeit highly variable, to slow cell proliferation of many cancer cell lines [58, 98, 99]. Some of these compounds have been tested for their efficacy in clinical trials for the treatment of cancers of various origins [58, 98, 99]. Many Plk1 inhibitors also block Plk3 activity to a certain extent . However, in contrast to Plk1, which plays a positive role in cell proliferation and tumor development, Plk3 is regarded as having a tumor-suppressing function [21, 30]. The latest study on the effect of Plk3 on tumor angiogenesis has provided results that are consistence with this notion . It is conceivable that a chemical compound that inhibits Plk3 activity specifically might have no beneficial effect to cancer treatment or management. Therefore, it is likely that the anti-tumor effect of those compounds which have an activity toward both Plk1 and Plk3 may result from Plk1 inhibition. Consequently, the discovery of Plk3 activators would be a more rational approach for anti-cancer drug development.
Targeting Plk3-mediated tumor angiogenesis may offer several advantages over Plk1 in cancer treatment. It have been shown that Plk1 knock-down via RNAi results in cell cycle arrest followed by apoptosis and that Plk1 knock-out mice is embryonically lethal [32, 100]. These results suggest that Plk1 is vital for normal cell proliferation and survival. Consequently, inhibition of Plk1 is likely to have a detrimental effect on normal cells in addition to cancer cells. In contrast, PLK3 ablation in mice does not noticeably perturb animal development except for enhanced tumor angiogenesis in mice of advanced age . Therefore targeting Plk3 would likely to have less side-effect in vivo. In addition, therapeutic intervention that activates Plk3 should lead to enhanced apoptosis in targeting cells as the effect has been observed in cells that overexpress Plk3 .
In spite of extensive research in the past which has yielded a sizable body of knowledge about the biochemical and cellular functions of Plk3, it remains as a protein kinase we know very little about, especially with regard to its physiological and patho-physiological roles. Mouse genetic studies have yielded valuable information about Plk3's role in suppressing tumor angiogenesis through negative regulation of the PI3K signaling network and the HIF-1 activity. Additional analyses are clearly needed to fully understand the physiological role of PLK3 in animal development and stress responses. Given its documented roles in suppressing cell proliferation in vitro and tumor development in vivo, Plk3 represents an attractive target for the development of anti-cancer compounds.
We thank co-workers in the laboratory for valuable discussions and suggestions. This study was supported in part by US Public Service Awards (CA113349 and ES019929), NIEHS Center grants (ES000260), and a key State project of China on iPS and stem cell research (2011ZX09102-010-04).
- Pugh CW, Ratcliffe PJ: Regulation of angiogenesis by hypoxia: role of the HIF system. Nat Med 2003, 9: 677–684. 10.1038/nm0603-677PubMedView ArticleGoogle Scholar
- Folkman J: Angiogenesis. Annu Rev Med 2006, 57: 1–18. 10.1146/annurev.med.57.121304.131306PubMedView ArticleGoogle Scholar
- Liao D, Johnson RS: Hypoxia: a key regulator of angiogenesis in cancer. Cancer Metastasis Rev 2007, 26: 281–290. 10.1007/s10555-007-9066-yPubMedView ArticleGoogle Scholar
- Folkman J: Tumor angiogenesis: therapeutic implications. N Engl J Med 1971, 285: 1182–1186. 10.1056/NEJM197111182852108PubMedView ArticleGoogle Scholar
- Semenza GL: Targeting HIF-1 for cancer therapy. Nat Rev Cancer 2003, 3: 721–732. 10.1038/nrc1187PubMedView ArticleGoogle Scholar
- Heath VL, Bicknell R: Anticancer strategies involving the vasculature. Nat Rev Clin Oncol 2009, 6: 395–404. 10.1038/nrclinonc.2009.52PubMedView ArticleGoogle Scholar
- Jain RK: Normalization of tumor vasculature: an emerging concept in antiangiogenic therapy. Science 2005, 307: 58–62. 10.1126/science.1104819PubMedView ArticleGoogle Scholar
- Zetter BR: Angiogenesis and tumor metastasis. Annu Rev Med 1998, 49: 407–424. 10.1146/annurev.med.49.1.407PubMedView ArticleGoogle Scholar
- Kaelin WG Jr, Ratcliffe PJ: Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Mol Cell 2008, 30: 393–402. 10.1016/j.molcel.2008.04.009PubMedView ArticleGoogle Scholar
- Ke Q, Costa M: Hypoxia-inducible factor-1 (HIF-1). Mol Pharmacol 2006, 70: 1469–1480. 10.1124/mol.106.027029PubMedView ArticleGoogle Scholar
- Yee-Koh M, Spivak-Kroizman TR, Powis G: HIF-1 regulation: not so easy come, easy go. Trends Biochem Sci 2008, 33: 526–534. 10.1016/j.tibs.2008.08.002PubMedView ArticleGoogle Scholar
- Flugel D, Gorlach A, Michiels C, Kietzmann T: Glycogen synthase kinase 3 phosphorylates hypoxia-inducible factor 1alpha and mediates its destabilization in a VHL-independent manner. Mol Cell Biol 2007, 27: 3253–3265. 10.1128/MCB.00015-07PubMed CentralPubMedView ArticleGoogle Scholar
- Mottet D, Dumont V, Deccache Y, Demazy C, Ninane N, Raes M, Michiels C: Regulation of hypoxia-inducible factor-1alpha protein level during hypoxic conditions by the phosphatidylinositol 3-kinase/Akt/glycogen synthase kinase 3beta pathway in HepG2 cells. J Biol Chem 2003, 278: 31277–31285. 10.1074/jbc.M300763200PubMedView ArticleGoogle Scholar
- Richard DE, Berra E, Gothie E, Roux D, Pouyssegur J: p42/p44 mitogen-activated protein kinases phosphorylate hypoxia-inducible factor 1alpha (HIF-1alpha) and enhance the transcriptional activity of HIF-1. J Biol Chem 1999, 274: 32631–32637. 10.1074/jbc.274.46.32631PubMedView ArticleGoogle Scholar
- Mylonis I, Chachami G, Samiotaki M, Panayotou G, Paraskeva E, Kalousi A, Georgatsou E, Bonanou S, Simos G: Identification of MAPK phosphorylation sites and their role in the localization and activity of hypoxia-inducible factor-1alpha. J Biol Chem 2006, 281: 33095–33106. 10.1074/jbc.M605058200PubMedView ArticleGoogle Scholar
- Mylonis I, Chachami G, Paraskeva E, Simos G: Atypical CRM1-dependent nuclear export signal mediates regulation of hypoxia-inducible factor-1alpha by MAPK. J Biol Chem 2008, 283: 27620–27627. 10.1074/jbc.M803081200PubMedView ArticleGoogle Scholar
- Xu D, Yao Y, Lu L, Costa M, Dai W: Plk3 functions as an essential component of the hypoxia regulatory pathway by direct phosphorylation of HIF-1alpha. J Biol Chem 2010, 285: 38944–38950. 10.1074/jbc.M110.160325PubMed CentralPubMedView ArticleGoogle Scholar
- Xu D, Yao Y, Jiang X, Lu L, Dai W: Regulation of PTEN stability and activity by Plk3. J Biol Chem 2010, 285: 39935–39942. 10.1074/jbc.M110.166462PubMed CentralPubMedView ArticleGoogle Scholar
- Yang Y, Bai J, Shen R, Brown SA, Komissarova E, Huang Y, Jiang N, Alberts GF, Costa M, Lu L, Winkles JA, Dai W: Polo-like kinase 3 functions as a tumor suppressor and is a negative regulator of hypoxia-inducible factor-1 alpha under hypoxic conditions. Cancer Res 2008, 68: 4077–4085. 10.1158/0008-5472.CAN-07-6182PubMed CentralPubMedView ArticleGoogle Scholar
- Fenton B, Glover DM: A conserved mitotic kinase active at late anaphase-telophase in syncytial Drosophila embryos. Nature 1993, 363: 637–640. 10.1038/363637a0PubMedView ArticleGoogle Scholar
- Dai W: Polo-like kinases, an introduction. Oncogene 2005, 24: 214–216. 10.1038/sj.onc.1208270PubMedView ArticleGoogle Scholar
- Andrysik Z, Bernstein WZ, Deng L, Myer DL, Li YQ, Tischfield JA, Stambrook PJ, Bahassi el M: The novel mouse Polo-like kinase 5 responds to DNA damage and localizes in the nucleolus. Nucleic Acids Res 2010, 38: 2931–43. 10.1093/nar/gkq011PubMed CentralPubMedView ArticleGoogle Scholar
- Lowery DM, Lim D, Yaffe MB: Structure and function of Polo-like kinases. Oncogene 2005, 24: 248–259. 10.1038/sj.onc.1208280PubMedView ArticleGoogle Scholar
- Archambault V, Glover DM: Polo-like kinases: conservation and divergence in their functions and regulation. Nat Rev Mol Cell Biol 2009, 10: 265–275. 10.1038/nrm2653PubMedView ArticleGoogle Scholar
- Winkles JA, Alberts GF: Differential regulation of polo-like kinase 1, 2, 3, and 4 gene expression in mammalian cells and tissues. Oncogene 2005, 24: 260–266. 10.1038/sj.onc.1208219PubMedView ArticleGoogle Scholar
- Seeburg DP, Pak D, Sheng M: Polo-like kinases in the nervous system. Oncogene 2005, 24: 292–298. 10.1038/sj.onc.1208277PubMedView ArticleGoogle Scholar
- Wang L, Payton R, Dai W, Lu L: Hyperosmotic stress-induced ATF-2 activation through Polo-like kinase 3 in human corneal epithelial cells. J Biol Chem 2011, 286: 1951–1958. 10.1074/jbc.M110.166009PubMed CentralPubMedView ArticleGoogle Scholar
- Wang L, Gao J, Dai W, Lu L: Activation of Polo-like kinase 3 by hypoxic stresses. J Biol Chem 2008, 283: 25928–25935. 10.1074/jbc.M801326200PubMed CentralPubMedView ArticleGoogle Scholar
- Wang L, Dai W, Lu L: Stress-induced c-Jun activation mediated by Polo-like kinase 3 in corneal epithelial cells. J Biol Chem 2007, 282: 32121–32127. 10.1074/jbc.M702791200PubMedView ArticleGoogle Scholar
- Eckerdt F, Yuan J, Strebhardt K: Polo-like kinases and oncogenesis. Oncogene 2005, 24: 267–276. 10.1038/sj.onc.1208273PubMedView ArticleGoogle Scholar
- Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I: Polo-like kinases (Plks) and cancer. Oncogene 2005, 24: 287–291. 10.1038/sj.onc.1208272PubMedView ArticleGoogle Scholar
- Lu LY, Wood JL, Minter-Dykhouse K, Ye L, Saunders TL, Yu X, Chen J: Polo-like kinase 1 is essential for early embryonic development and tumor suppression. Mol Cell Biol 2008, 28: 6870–6876. 10.1128/MCB.00392-08PubMed CentralPubMedView ArticleGoogle Scholar
- Li B, Ouyang B, Pan H, Reissmann PT, Slamon DJ, Arceci R, Lu L, Dai W: Prk, a cytokine-inducible human protein serine/threonine kinase whose expression appears to be down-regulated in lung carcinomas. J Biol Chem 1996, 271: 19402–19408. 10.1074/jbc.271.32.19402PubMedView ArticleGoogle Scholar
- Dai W, Li Y, Ouyang B, Pan H, Reissmann P, Li J, Wiest J, Stambrook P, Gluckman JL, Noffsinger A, Bejarano P: PRK, a cell cycle gene localized to 8p21, is downregulated in head and neck cancer. Genes Chromosomes Cancer 2000, 27: 332–336. 10.1002/(SICI)1098-2264(200003)27:3<332::AID-GCC15>3.0.CO;2-KPubMedView ArticleGoogle Scholar
- Donohue PJ, Alberts GF, Guo Y, Winkles JA: Identification by targeted differential display of an immediate early gene encoding a putative serine/threonine kinase. J Biol Chem 1995, 270: 10351–10357. 10.1074/jbc.270.17.10351PubMedView ArticleGoogle Scholar
- Bahassi el M, Conn CW, Myer DL, Hennigan RF, McGowan CH, Sanchez Y, Stambrook PJ: Mammalian Polo-like kinase 3 (Plk3) is a multifunctional protein involved in stress response pathways. Oncogene 2002, 21: 6633–6640. 10.1038/sj.onc.1205850PubMedView ArticleGoogle Scholar
- Chase D, Feng Y, Hanshew B, Winkles JA, Longo DL, Ferris DK: Expression and phosphorylation of fibroblast-growth-factor-inducible kinase (Fnk) during cell-cycle progression. Biochem J 1998,333(Pt 3):655–660.PubMed CentralPubMedView ArticleGoogle Scholar
- Zimmerman WC, Erikson RL: Polo-like kinase 3 is required for entry into S phase. Proc Natl Acad Sci USA 2007, 104: 1847–1852. 10.1073/pnas.0610856104PubMed CentralPubMedView ArticleGoogle Scholar
- Xie S, Wu H, Wang Q, Cogswell JP, Husain I, Conn C, Stambrook P, Jhanwar-Uniyal M, Dai W: Plk3 functionally links DNA damage to cell cycle arrest and apoptosis at least in part via the p53 pathway. J Biol Chem 2001, 276: 43305–43312. 10.1074/jbc.M106050200PubMedView ArticleGoogle Scholar
- Xie S, Wu H, Wang Q, Kunicki J, Thomas RO, Hollingsworth RE, Cogswell J, Dai W: Genotoxic stress-induced activation of Plk3 is partly mediated by Chk2. Cell Cycle 2002, 1: 424–429. 10.4161/cc.1.6.271PubMedView ArticleGoogle Scholar
- Kis E, Szatmari T, Keszei M, Farkas R, Esik O, Lumniczky K, Falus A, Safrany G: Microarray analysis of radiation response genes in primary human fibroblasts. Int J Radiat Oncol Biol Phys 2006, 66: 1506–1514. 10.1016/j.ijrobp.2006.08.004PubMedView ArticleGoogle Scholar
- Xie S, Xie B, Lee MY, Dai W: Regulation of cell cycle checkpoints by polo-like kinases. Oncogene 2005, 24: 277–286. 10.1038/sj.onc.1208218PubMedView ArticleGoogle Scholar
- Wang Q, Xie S, Chen J, Fukasawa K, Naik U, Traganos F, Darzynkiewicz Z, Jhanwar-Uniyal M, Dai W: Cell cycle arrest and apoptosis induced by human Polo-like kinase 3 is mediated through perturbation of microtubule integrity. Mol Cell Biol 2002, 22: 3450–3459. 10.1128/MCB.22.10.3450-3459.2002PubMed CentralPubMedView ArticleGoogle Scholar
- Ouyang B, Li W, Pan H, Meadows J, Hoffmann I, Dai W: The physical association and phosphorylation of Cdc25C protein phosphatase by Prk. Oncogene 1999, 18: 6029–6036. 10.1038/sj.onc.1202983PubMedView ArticleGoogle Scholar
- Bahassi el M, Hennigan RF, Myer DL, Stambrook PJ: Cdc25C phosphorylation on serine 191 by Plk3 promotes its nuclear translocation. Oncogene 2004, 23: 2658–2663. 10.1038/sj.onc.1207425PubMedView ArticleGoogle Scholar
- Lopez-Girona A, Furnari B, Mondesert O, Russell P: Nuclear localization of Cdc25 is regulated by DNA damage and a 14–3-3 protein. Nature 1999, 397: 172–175. 10.1038/16488PubMedView ArticleGoogle Scholar
- Zimmerman WC, Erikson RL: Finding Plk3. Cell Cycle 2007, 6: 1314–1318. 10.4161/cc.6.11.4275PubMedView ArticleGoogle Scholar
- Iida M, Matsuda M, Komatani H: Plk3 phosphorylates topoisomerase IIalpha at Thr(1342), a site that is not recognized by Plk1. Biochem J 2008, 411: 27–32. 10.1042/BJ20071394PubMedView ArticleGoogle Scholar
- Wang JC: Cellular roles of DNA topoisomerases: a molecular perspective. Nat Rev Mol Cell Biol 2002, 3: 430–440. 10.1038/nrm831PubMedView ArticleGoogle Scholar
- Lopez-Sanchez I, Sanz-Garcia M, Lazo PA: Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation. Mol Cell Biol 2009, 29: 1189–1201. 10.1128/MCB.01341-08PubMed CentralPubMedView ArticleGoogle Scholar
- Ruan Q, Wang Q, Xie S, Fang Y, Darzynkiewicz Z, Guan K, Jhanwar-Uniyal M, Dai W: Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle. Exp Cell Res 2004, 294: 51–59. 10.1016/j.yexcr.2003.10.022PubMedView ArticleGoogle Scholar
- Xie S, Wang Q, Ruan Q, Liu T, Jhanwar-Uniyal M, Guan K, Dai W: MEK1-induced Golgi dynamics during cell cycle progression is partly mediated by Polo-like kinase-3. Oncogene 2004, 23: 3822–3829. 10.1038/sj.onc.1207479PubMedView ArticleGoogle Scholar
- Conn CW, Hennigan RF, Dai W, Sanchez Y, Stambrook PJ: Incomplete cytokinesis and induction of apoptosis by overexpression of the mammalian polo-like kinase, Plk3. Cancer Res 2000, 60: 6826–6831.PubMedGoogle Scholar
- Li Z, Niu J, Uwagawa T, Peng B, Chiao PJ: Function of polo-like kinase 3 in NF-kappaB-mediated proapoptotic response. J Biol Chem 2005, 280: 16843–16850. 10.1074/jbc.M410119200PubMedView ArticleGoogle Scholar
- Xie S, Wang Q, Wu H, Cogswell J, Lu L, Jhanwar-Uniyal M, Dai W: Reactive oxygen species-induced phosphorylation of p53 on serine 20 is mediated in part by polo-like kinase-3. J Biol Chem 2001, 276: 36194–36199. 10.1074/jbc.M104157200PubMedView ArticleGoogle Scholar
- Kruse JP, Gu W: Modes of p53 regulation. Cell 2009, 137: 609–622. 10.1016/j.cell.2009.04.050PubMed CentralPubMedView ArticleGoogle Scholar
- Dai W, Liu T, Wang Q, Rao CV, Reddy BS: Down-regulation of PLK3 gene expression by types and amount of dietary fat in rat colon tumors. Int J Oncol 2002, 20: 121–126.PubMedGoogle Scholar
- Dai W, Yang Y, Jiang N: Plks as Novel Targets for Cancer Drug Design. In Cancer Drug Discovery and Development. Edited by: Dai W. Totowa: Humana Press; 2008:271–290.Google Scholar
- Wiest J, Clark AM, Dai W: Intron/exon organization and polymorphisms of the PLK3/PRK gene in human lung carcinoma cell lines. Genes Chromosomes Cancer 2001, 32: 384–389. 10.1002/gcc.1204PubMedView ArticleGoogle Scholar
- Bockmuhl U, Wolf G, Schmidt S, Schwendel A, Jahnke V, Dietel M, Petersen I: Genomic alterations associated with malignancy in head and neck cancer. Head Neck 1998, 20: 145–151. 10.1002/(SICI)1097-0347(199803)20:2<145::AID-HED8>3.0.CO;2-2PubMedView ArticleGoogle Scholar
- Spurr NK, Blanton S, Bookstein R, Clarke R, Cottingham R, Daiger S, Drayna D, Faber P, Horrigan S, Kas K, et al.: Report and abstracts of the second international workshop on human chromosome 8 mapping 1994. Oxford, United Kingdom, September 16–18, 1994. Cytogenet Cell Genet 1995, 68: 147–164. 10.1159/000133908PubMedView ArticleGoogle Scholar
- Wang GL, Jiang BH, Rue EA, Semenza GL: Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA 1995, 92: 5510–5514. 10.1073/pnas.92.12.5510PubMed CentralPubMedView ArticleGoogle Scholar
- Cully M, You H, Levine AJ, Mak TW: Beyond PTEN mutations: the PI3K pathway as an integrator of multiple inputs during tumorigenesis. Nat Rev Cancer 2006, 6: 184–192. 10.1038/nrc1819PubMedView ArticleGoogle Scholar
- Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang SI, Puc J, Miliaresis C, Rodgers L, McCombie R, Bigner SH, Giovanella BC, Ittmann M, Tycko B, Hibshoosh H, Wigler MH, Parsons R: PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 1997, 275: 1943–1947. 10.1126/science.275.5308.1943PubMedView ArticleGoogle Scholar
- Whitman M, Downes CP, Keeler M, Keller T, Cantley L: Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate. Nature 1988, 332: 644–646. 10.1038/332644a0PubMedView ArticleGoogle Scholar
- Liu P, Cheng H, Roberts TM, Zhao JJ: Targeting the phosphoinositide 3-kinase pathway in cancer. Nat Rev Drug Discov 2009, 8: 627–644. 10.1038/nrd2926PubMed CentralPubMedView ArticleGoogle Scholar
- Hamada K, Sasaki T, Koni PA, Natsui M, Kishimoto H, Sasaki J, Yajima N, Horie Y, Hasegawa G, Naito M, Miyazaki J, Suda T, Itoh H, Nakao K, Mak TW, Nakano T, Suzuki A: The PTEN/PI3K pathway governs normal vascular development and tumor angiogenesis. Genes Dev 2005, 19: 2054–2065. 10.1101/gad.1308805PubMed CentralPubMedView ArticleGoogle Scholar
- Jiang BH, Zheng JZ, Aoki M, Vogt PK: Phosphatidylinositol 3-kinase signaling mediates angiogenesis and expression of vascular endothelial growth factor in endothelial cells. Proc Natl Acad Sci USA 2000, 97: 1749–1753. 10.1073/pnas.040560897PubMed CentralPubMedView ArticleGoogle Scholar
- Ma J, Sawai H, Ochi N, Matsuo Y, Xu D, Yasuda A, Takahashi H, Wakasugi T, Takeyama H: PTEN regulates angiogenesis through PI3K/Akt/VEGF signaling pathway in human pancreatic cancer cells. Mol Cell Biochem 2009, 331: 161–171. 10.1007/s11010-009-0154-xPubMedView ArticleGoogle Scholar
- Wen S, Stolarov J, Myers MP, Su JD, Wigler MH, Tonks NK, Durden DL: PTEN controls tumor-induced angiogenesis. Proc Natl Acad Sci USA 2001, 98: 4622–4627. 10.1073/pnas.081063798PubMed CentralPubMedView ArticleGoogle Scholar
- Ueda S, Basaki Y, Yoshie M, Ogawa K, Sakisaka S, Kuwano M, Ono M: PTEN/Akt signaling through epidermal growth factor receptor is prerequisite for angiogenesis by hepatocellular carcinoma cells that is susceptible to inhibition by gefitinib. Cancer Res 2006, 66: 5346–5353. 10.1158/0008-5472.CAN-05-3684PubMedView ArticleGoogle Scholar
- Sutherland C, Leighton IA, Cohen P: Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling. Biochem J 1993,296(Pt 1):15–19.PubMed CentralPubMedView ArticleGoogle Scholar
- Stambolic V, Woodgett JR: Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Biochem J 1994,303(Pt 3):701–704.PubMed CentralPubMedView ArticleGoogle Scholar
- Cross DA, Alessi DR, Cohen P, Andjelkovich M, Hemmings BA: Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 1995, 378: 785–789. 10.1038/378785a0PubMedView ArticleGoogle Scholar
- Gericke A, Munson M, Ross AH: Regulation of the PTEN phosphatase. Gene 2006, 374: 1–9.PubMedView ArticleGoogle Scholar
- Miller SJ, Lou DY, Seldin DC, Lane WS, Neel BG: Direct identification of PTEN phosphorylation sites. FEBS Lett 2002, 528: 145–153. 10.1016/S0014-5793(02)03274-XPubMedView ArticleGoogle Scholar
- Torres J, Pulido R: The tumor suppressor PTEN is phosphorylated by the protein kinase CK2 at its C terminus. Implications for PTEN stability to proteasome-mediated degradation. J Biol Chem 2001, 276: 993–998. 10.1074/jbc.M009134200PubMedView ArticleGoogle Scholar
- Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T: Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta. J Biol Chem 2005, 280: 35195–35202. 10.1074/jbc.M503045200PubMedView ArticleGoogle Scholar
- Mehenni H, Lin-Marq N, Buchet-Poyau K, Reymond A, Collart MA, Picard D, Antonarakis SE: LKB1 interacts with and phosphorylates PTEN: a functional link between two proteins involved in cancer predisposing syndromes. Hum Mol Genet 2005, 14: 2209–2219. 10.1093/hmg/ddi225PubMedView ArticleGoogle Scholar
- Valiente M, Andres-Pons A, Gomar B, Torres J, Gil A, Tapparel C, Antonarakis SE, Pulido R: Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases. J Biol Chem 2005, 280: 28936–28943. 10.1074/jbc.M504761200PubMedView ArticleGoogle Scholar
- Lu Y, Yu Q, Liu JH, Zhang J, Wang H, Koul D, McMurray JS, Fang X, Yung WK, Siminovitch KA, Mills GB: Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades. J Biol Chem 2003, 278: 40057–40066. 10.1074/jbc.M303621200PubMedView ArticleGoogle Scholar
- Li Z, Dong X, Wang Z, Liu W, Deng N, Ding Y, Tang L, Hla T, Zeng R, Li L, Wu D: Regulation of PTEN by Rho small GTPases. Nat Cell Biol 2005, 7: 399–404. 10.1038/ncb1236PubMedView ArticleGoogle Scholar
- Adey NB, Huang L, Ormonde PA, Baumgard ML, Pero R, Byreddy DV, Tavtigian SV, Bartel PL: Threonine phosphorylation of the MMAC1/PTEN PDZ binding domain both inhibits and stimulates PDZ binding. Cancer Res 2000, 60: 35–37.PubMedGoogle Scholar
- Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers WR: Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex. J Biol Chem 2001, 276: 48627–48630. 10.1074/jbc.C100556200PubMedView ArticleGoogle Scholar
- Tolkacheva T, Boddapati M, Sanfiz A, Tsuchida K, Kimmelman AC, Chan AM: Regulation of PTEN binding to MAGI-2 by two putative phosphorylation sites at threonine 382 and 383. Cancer Res 2001, 61: 4985–4989.PubMedGoogle Scholar
- Odriozola L, Singh G, Hoang T, Chan AM: Regulation of PTEN activity by its carboxyl-terminal autoinhibitory domain. J Biol Chem 2007, 282: 23306–23315. 10.1074/jbc.M611240200PubMedView ArticleGoogle Scholar
- Rahdar M, Inoue T, Meyer T, Zhang J, Vazquez F, Devreotes PN: A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN. Proc Natl Acad Sci USA 2009, 106: 480–485. 10.1073/pnas.0811212106PubMed CentralPubMedView ArticleGoogle Scholar
- Vazquez F, Ramaswamy S, Nakamura N, Sellers WR: Phosphorylation of the PTEN tail regulates protein stability and function. Mol Cell Biol 2000, 20: 5010–5018. 10.1128/MCB.20.14.5010-5018.2000PubMed CentralPubMedView ArticleGoogle Scholar
- Carracedo A, Alimonti A, Pandolfi PP: PTEN level in tumor suppression: how much is too little? Cancer Res 2011, 71: 629–633. 10.1158/0008-5472.CAN-10-2488PubMed CentralPubMedView ArticleGoogle Scholar
- Trotman LC, Niki M, Dotan ZA, Koutcher JA, Di Cristofano A, Xiao A, Khoo AS, Roy-Burman P, Greenberg NM, Van Dyke T, Cordon-Cardo C, Pandolfi PP: Pten dose dictates cancer progression in the prostate. PLoS Biol 2003, 1: E59.PubMed CentralPubMedView ArticleGoogle Scholar
- Kwabi-Addo B, Giri D, Schmidt K, Podsypanina K, Parsons R, Greenberg N, Ittmann M: Haploinsufficiency of the Pten tumor suppressor gene promotes prostate cancer progression. Proc Natl Acad Sci USA 2001, 98: 11563–11568. 10.1073/pnas.201167798PubMed CentralPubMedView ArticleGoogle Scholar
- Casamayor A, Morrice NA, Alessi DR: Phosphorylation of Ser-241 is essential for the activity of 3-phosphoinositide-dependent protein kinase-1: identification of five sites of phosphorylation in vivo. Biochem J 1999,342(Pt 2):287–292.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang X, Trotman LC, Koppie T, Alimonti A, Chen Z, Gao Z, Wang J, Erdjument-Bromage H, Tempst P, Cordon-Cardo C, Pandolfi PP, Jiang X: NEDD4–1 is a proto-oncogenic ubiquitin ligase for PTEN. Cell 2007, 128: 129–139. 10.1016/j.cell.2006.11.039PubMed CentralPubMedView ArticleGoogle Scholar
- Wu W, Wang X, Zhang W, Reed W, Samet JM, Whang YE, Ghio AJ: Zinc-induced PTEN protein degradation through the proteasome pathway in human airway epithelial cells. J Biol Chem 2003, 278: 28258–28263. 10.1074/jbc.M303318200PubMedView ArticleGoogle Scholar
- Davies SP, Reddy H, Caivano M, Cohen P: Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J 2000, 351: 95–105. 10.1042/0264-6021:3510095PubMed CentralPubMedView ArticleGoogle Scholar
- Cohen P: Protein kinases-the major drug targets of the twenty-first century? Nat Rev Drug Discov 2002, 1: 309–315. 10.1038/nrd773PubMedView ArticleGoogle Scholar
- Noble ME, Endicott JA, Johnson LN: Protein kinase inhibitors: insights into drug design from structure. Science 2004, 303: 1800–1805. 10.1126/science.1095920PubMedView ArticleGoogle Scholar
- Strebhardt K: Multifaceted polo-like kinases: drug targets and antitargets for cancer therapy. Nat Rev Drug Discov 2010, 9: 643–660. 10.1038/nrd3184PubMedView ArticleGoogle Scholar
- Schoffski P: Polo-like kinase (PLK) inhibitors in preclinical and early clinical development in oncology. Oncologist 2009, 14: 559–570. 10.1634/theoncologist.2009-0010PubMedView ArticleGoogle Scholar
- Gleixner KV, Ferenc V, Peter B, Gruze A, Meyer RA, Hadzijusufovic E, Cerny-Reiterer S, Mayerhofer M, Pickl WF, Sillaber C, Valent P: Polo-like kinase 1 (Plk1) as a novel drug target in chronic myeloid leukemia: overriding imatinib resistance with the Plk1 inhibitor BI 2536. Cancer Res 2010, 70: 1513–23. 10.1158/0008-5472.CAN-09-2181PubMedView ArticleGoogle Scholar
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