Fig. 5
The Treg and IL-10 in shaping the neutrophil phenotype. a Clustering of selected differentially expressed genes encoding receptor ligands in Treg isolated from control (Treg_Ctrl) and TCL1 leukemia-bearing mice (Treg_TCL1) 14 days after TCL1 leukemia (CD5+ CD19+) cell inoculation, when there were 10–30% leukemic cells among WBC in the blood. b Scheme presenting the timeline of the experiment. Treg were depleted in DEREG mice using diphtheria toxin (DT) one day before TCL1 cells injection. DT administration was repeated every 4 days. Anti-IL10 antibody (α-IL10) or the appropriate isotype control (ISO) were administered every 3 days starting from Day 4. The phenotype of splenic neutrophils was analyzed by flow cytometry two weeks after TCL1 leukemia (CD5+ CD19+) cells inoculation, when there were 10–30% leukemic cells among WBC in the blood. c The expression of the selected surface markers of splenic granulocytes (CD11b+ Ly6G+) isolated from TCL1 leukemia-bearing DEREG mice untreated (TCL1) or treated with DT (TCL1 DT) and treated with anti-IL10 antibody (α-IL10) or isotype control (ISO), MFI – mean fluorescence intensity; each point represents an individual mouse, n = 4–5, Mann‒Whitney U test, ns – not significant, *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001