Fig. 4

Phenotype of neutrophils in TCL1 leukemia-bearing mice upon induction of the antileukemic immune response by Treg depletion. a Scheme presenting the timeline of the experiment. Regulatory T cells (Treg) were depleted in DEREG mice using diphtheria toxin (DT) one day before TCL1 cells (CD5⁺ CD19⁺) injection. DT administration was repeated every 4 days. The phenotype of splenic neutrophils was analyzed by flow cytometry two weeks after leukemic cell inoculation, when there were 10–40% leukemic (CD5⁺ CD19⁺) cells among WBC in the blood. b, c The expression of the selected surface markers of splenic granulocytes (CD11b⁺ Ly6G⁺) isolated from the control (CTR) and TCL1 leukemia-bearing DEREG mice untreated (TCL1) or treated with DT (TCL1 DT), MFI – mean fluorescence intensity; the graphs presented in the upper panels show results from two independent experiments; each point represents an individual mouse, n = 4–7, Mann‒Whitney U test, ns – not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Representative dot plots are shown in the lower panels