Fig. 2
From: Identification of a novel NPM1 mutation in acute myeloid leukemia
A Representative images show cellular localization of NPM1 (green) in blasts from typical AML patients, with NPM1 wildtype, NPM1 type A mutation and this new exon 5 mutation (NPM1_MutSong), respectively. DAPI was used for nuclei staining (blue). Fibrillarin was used for nucleolus staining. White arrows indicated the predominate cytoplasmic location of NPM1 in mutation NPM1_MutSong. Images were collected by Nikon eclipse Ti2 confocal microscope; magnification, × 600. B Immunohistochemical staining of bone marrow biopsies from Pt. Song with the new NPM1 exon 5 mutation. Representative images show diffuse infiltration by leukemia blasts (hematoxylin and eosin [HE], left), with aberrant cytoplasmic localization of NPM1 (right, brown). Images were collected by Pannoramic 250 FLASH; magnification, × 630. C Western blot analysis with anti-GFP antibody (left, ~ 27 kD) or anti-NPM1 antibody (right, ~ 38 kD) both recognizing NPM1 proteins in HEK-293T cells overexpressing GFP-NPM1 exon 5 fusion protein (~ 61 kD). D HEK-293T cells overexpressing the new GFP-NPM1 fusion protein show the aberrant cytoplasmic subcellular location, concomitantly, with nucleolar location. Upon treatment with leptomycin B, NPM1 was re-localized in nucleus. Images were collected by Olympus IX71 confocal microscope; magnification, × 200