Fig. 5
From: The PTX3/TLR4 autocrine loop as a novel therapeutic target in triple negative breast cancer
PTX3 activates TLR4/IRAK1 signaling in TNBC. (A) GSEA of MDA-MB-231 (shNT vs. shPTX3) genes associated with TLR4 signaling (left panel) and inflammatory response to LPS (right panel). (B) Bio-Plex assay of secreted pro-inflammatory cytokines from MDA-MB-231 cells (shPTX3 vs. shNT). (C) Western blot analysis of MDA-MB-231 shNT and shPTX3 cells treated or not with 10 µg/ml of anti-PTX3 or anti-TLR4 antibodies for 6 h. (D) Western blot analysis of MDA-MB-231 shNT and shPTX3 cells treated or not with 100 nM of recombinant human PTX3 (rhPTX3) for 24 h. (E) Immunohistochemical analysis of MDA-MB-231 shNT and shPTX3 tumors. Scale bar: 50 μm
Data are the mean ± SEM, experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001