Fig. 2
From: The PTX3/TLR4 autocrine loop as a novel therapeutic target in triple negative breast cancer
Effects of PTX3 silencing in MDA-MB-231 cells. (A) Western blot analysis of PTX3 silencing. (B) Cell proliferation assay by viable cell counting through cytofluorimetric analysis. (C) Gene set enrichment analysis (GSEA) of genes associated with proliferation. (D) Colony formation assay. White bars indicate the number of colonies, grey bars indicate the absorbance after crystal violet staining and solubilization of the colonies. (E) Soft agar assay. (F) Wound healing assay. (G) Percentage of ALDH-positive cells quantified by cytofluorimetric analysis. Green dots represent DEAB treated control cell population, black dots represent cell population not treated with DEAB. Gates have been set according to DEAB treated cell population. (H) Tumor sphere formation assay. (I) GSEA of genes associated with BC progenitors
Data are the mean ± SEM, experiments were performed in triplicate. In box and whiskers graphs, boxes extend from the 25th to the 75th percentiles, lines indicate the median values, and whiskers indicate the range of values. *p < 0.05, **p < 0.01, ***p < 0.001