Fig. 1
From: The PTX3/TLR4 autocrine loop as a novel therapeutic target in triple negative breast cancer
PTX3 expression in human BC. (A) PTX3 mRNA expression in patient-derived tumor samples by GOBO database. (B) Western blot analysis of PTX3 expression in patient-derived tumor samples. (C) Correlation of PTX3 expression with tumor grade by GOBO database. D) Single cell analyses of TNBC samples (left panel) and PTX3 expression in each cell population (right panel). (E) RNAscope analysis for PTX3 expression (red staining) in patient-derived tumor samples. Tumor cells are identified by immunohistochemical staining for pan cytokeratin (CK) expression (blue membrane staining). (F) Data from GOBO database about PTX3 expression in BC cell lines at mRNA level. (G) Data from EMBL-EBI expression atlas database about PTX3 expression in BC cell lines at mRNA level. FPKM: Fragments Per Kilobase of transcript per Million map reads. (H) PTX3 expression by qPCR and Western blot analyses and PTX3 secreted levels by ELISA from several BC cell lines