Fig. 7

COP1 promotes CRC partially by degrading UTX. A and B IB analysis (A) and representative IHC staining (B) of the indicated proteins in intestinal epithelial cells of WT and Cop1^−/+ mice. Scale bar, 20 μm. C Significant inverse correlation (r = -0.257, P < 0.001) between COP1 and UTX expression was determined by Pearson’s correlation analysis of CRC tissue microarray. D Representative IHC images of COP1 and UTX staining in CRC samples. Scale bar, 100 μm (left) and 20 μm (right). E and F Xenograft tumorigenesis of HCT116 cells with ectopic HA-COP1 or Flag-UTX-mutant (VP-AA) expression (n = 7, respectively). Scale bar, 1 cm. G Tumor weight of nude mice in different groups. H IB analysis of indicated proteins in tumors from different groups (n = 3). I Graphic model of dysregulated UTX contributing to CRC tumorigenesis. Data information: In (F and G), data are presented as mean ± SEM (one-way ANOVA with Dunnett’s multiple comparisons test). ^***P < 0.001