Fig. 6

COP1 is an oncogenic protein in CRC. A Representative IHC images of COP1 staining in paired CRC and surrounding normal tissues. Scale bar, 100 μm (left) and 20 μm (right). B COP1 protein expression assessed by IHC scores of TMA in paired CRC and surrounding normal tissues (N = 104). C IB analyses of WCL (left) and statistical analysis of colony formation assays (right) in HCT116 cells treated with siCOP1 or NC. D IB analyses of WCL (left) and statistical analysis of colony formation assays (right) in LoVo cells treated with siCOP1 or NC. E and F Representative macroscopic (E) and H&E staining images (F) of large intestines from WT (n = 7) and Cop1^−/+ (n = 8) mice at the end of the AOM/DSS protocol. Tumors were surrounded by dotted lines in representative images of H&E staining. Scale bar in (E), 5000 μm. Scale bar in (F), 1000 μm (upper), and 20 μm (bottom). G Tumor number and load from large intestines between WT and Cop1^−/+ mice. Data information: In (B, C, D and G), data are presented as mean ± SEM (two-tailed Student’s t-test). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001