Fig. 3

UTX inhibits tumorigenesis by upregulating the expression of EMP1 and AUTS2 in HCT116 cells. A IB of whole cell lysate (WCL) derived from HCT116 cells infected with lentiviruses expressing pLKO.1 or shUTX. B Cell growth curve analysis of HCT116 cells infected with indicated lentiviruses. C Statistical analysis of soft agar assays in HCT116 cells infected with indicated lentiviruses. D Workflow for screening potential tumor suppressive target genes of UTX. E qPCR confirmation of UTX, EMP1, and AUTS2 expression changes in HCT116 cells transfected with siUTX or negative control (NC). F qPCR detection of Utx, Emp1, and Auts2 expression in intestinal epithelial cells from WT and Utx^−/y mice. G IB analysis of WCL from HCT116 cells infected with indicated lentiviruses.H Apoptosis of HCT116 cells infected with indicated lentiviruses was detected by TUNEL assay and observed under light microscopy. Scale bar, 50 μm. I Statistical analysis of TUNEL-positive cells in different groups. Data information: In (B, C, E, and F), data are represented as means ± SEM (two-tailed Student’s t-test). In (I), data are represented as means ± SEM (one-way ANOVA with Dunnett’s multiple comparisons test). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001