Fig. 2

Conditional deletion of Utx in intestinal epithelial cells increases susceptibility to AOM/DSS-induced colorectal tumorigenesis. A Schematic model of the AOM/DSS protocol used to induce colorectal tumors in C57BL/6 mice. i.p., intraperitoneal. B Relative body weight of WT and Utx^−/x/Utx^−/y was recorded during AOM/DSS treatment C and D Representative macroscopic (C) and H & E staining images (D) of large intestines from WT (n = 10), Utx^−/x (n = 6), and Utx^−/y (n = 5) mice at the end of the AOM/DSS protocol. Tumors were surrounded by dotted lines in representative images of H&E staining. Scale bar in (C), 5000 μm. Scale bar in (D), 1000 μm (left), and 20 μm (right), respectively. E and F Tumor number, tumor load (E), and size distribution of tumors (F) from large intestines of WT (n = 10), Utx^−/x (n = 6), and Utx^−/y (n = 5) mice. G Representative IHC images of Ki67 staining in large intestines of WT and Utx^−/y mice post AOM/DSS-induced CRC tumorigenesis. Scale bar, 20 μm. Data information: In (B and E), data are presented as mean ± SEM (one-way ANOVA with Dunnett’s multiple comparisons test). In (F), data are presented as rates (chi-square test). ns, non-significance, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001