Fig. 5

PE8T CAR-T cells harnessed tumor and prolonged survival in vivo compared with 2G CAR-T cells. A. Schematic representation of the experimental procedure for tumor challenge, T cell adoptive transfer, cytokines detection, and in vivo imaging. B. Representative bioluminescent images of tumor over time. C. The logarithm of total body flux (photons/s) for each mouse was quantified and averaged per group (mean ± SEM, n = 6). D. Kaplan–Meier survival analysis of NALM-6-PDL1-GFP-luc challenged mice. Overall survival curves were plotted using the Kaplan–Meier method and compared utilizing the log-rank (Mantel–Cox) test (n = 6). *P < 0.05; **P < 0.01; ***P < 0.005. E. On day 5, 9, 16, 20, blood was collected from one group mixed to detect the concentration of IFN-γ and IL-2 using an ELISA-kit (mean ± SD, n = 2)