Fig. 3

The PEPT CAR-T cells enhanced anti-tumor activity without off-target toxicity to PDL1 in vivo. A. Schematic representation of the experimental procedure for tumor challenge, T cell adoptive transfer, cytokine detection, and in vivo imaging. B. Representative bioluminescent images of tumor growth over time. C. Total body flux (photons/s) for each mouse was quantified and averaged per group. Error bars represent mean ± SEM.D. Kaplan-Meier survival analysis of NALM-6-PDL1-GFP-luc challenged mice. Overall survival curves were plotted using the Kaplan-Meier method and compared using the log-rank (Mantel-Cox) test (n = 4 or 5; * P < 0.05). E. Murine PDL1/2 expression in different tissues of NSG mice stained with human PDL1/2 antibodies by flow cytometry. F. On day 4, blood was collected from the tail vein. The samples from all mice of each group were pooled, and plasma was isolated. The concentration of IFN-γ was detected using an ELISA-kit in duplicate