Fig. 4

Activity of optimized 42105-74bbz CAR-T cells against primary MCL patient lymphoma cells. A Expression of CD74 (gray) on MCL patients’ lymphoma cells compared to isotype control (white). The data presented are one representative of three independent experiments. B Susceptibility of five MCL patient lymphoma cells to the 42105-74bbz CAR-T cells. MCL patient cells were used as target cells in the co-culture of optimized 74bbz CAR-T cells at E:T ratio of 5:1 for 24 h. Results were presented as mean ± SEM from three independent experiments. ** p < 0.01; ***p < 0.001. C Antigenic dependence of CD74 on the specific lysis of 42105-74bbz CAR-T cells. The CD74 surface antigen density estimated by MESF and specific lysis values of JeKo-1, Mino, UPN-1, Granta-519, Z138 (left), and Patient 1–5 (right) including negative control SUDHL-1 were used to perform the correlation analysis for the correlation coefficient r and the p-value