Fig. 3

Cytotoxicity and IFN-γ production of optimized 42105-74bbz CAR-T cells against MCL cells. A Expression of CD74 on MCL cell lines. The data presented are one representative of three independent experiments. Unstained and isotype controls were used as a staining negative control. B Cytotoxicity assay of optimized 42105-74bbz CAR-T cells against 6 different MCL cell lines. Untransduced T cells (UTT, red circle) or optimized 42105-74bbz CAR-T cells (blue, triangles) were co-cocultured with target cells at E:T ratio of 5:1 for 24 h before the specific lysis was determined. CD74− T cell lymphoma cell line SUDHL-1 was used as a negative control. Each dot represents one experimental result from each healthy donor CAR-T cells. C IFN-γ production from 42105-74bbz CAR-T cells after co-cultured with indicated cell for 24 h. Effector alone (EA) was used as the negative control. Results were presented as mean ± SEM from three independent experiments. * p < 0.05; ** p < 0.01; ***p < 0.001