Fig. 2

42105-74bbz CAR-T cells induced the highest specific lysis among mutants against Mino and JeKo-1 cells. A Three 74bbz CAR mutants were expressed on primary CD4/8 T cells (n = 3) and used as effector cells in the co-culture of Mino (left) and JeKo-1 (right). Mutants 543, 5311 and 42105 were compared to the parent 74bbz CAR-T cells. B 42105-74bbz CAR-T cells had the highest specific lysis signal-to-noise as determined by the specific lysis on CD74+ Mino cells over the CD74- SUDHL-1 cells. The ratios from all three mutants were compared to that of the parent. Data are mean ± S.E.M. from three independent experiments. * p < 0.05; ** p < 0.01; ***p < 0.001