Fig. 6

LSD1 and proteasome inhibition activate the DNA damage pathway. A U266 cells were treated with 2.5 nM CFZ, 1 µM SP2509, 1 µM GSK-LSD1, or the combinations. Cell pellets were collected at 48 h post-treatment: Expression of the indicated proteins was analyzed by Western blot. β-actin and vinculin were used as loading controls. B KMS28-TTA_shLSD1-D6 cells were pre-treated with DOX for 5 days and then treated with CFZ. Cell pellets were collected 72 h post-CFZ treatment, and protein expression was analyzed by Western blot. β-actin and vinculin were used as loading controls