Table 1 Culture conditions for different tumor organoids

Medium

Advance DMEM/F12

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

HEPES 10 mM

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

Glutamax 2 mM

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

Antibiotics/antimycotics (1%)

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

√

Cytokines

rhR-Spondin-1 (ng/ml)

500

250

1000

250

500

500

250

125

250

100

rhNoggin (ng/ml)

100

1000

100

100

100

25

100

100

40

rmNoggin (ng/ml)

100

100

100

rhFGF basic (ng/ml)

12.5

20

1

50

5

rhFGF-7 (ng/ml)

5

25

25

25

rhFGF-10 (ng/ml)

50

20

100

100

100

100

100

10

100

10

rhIGF (ng/ml)

100

rhEGF (ng/ml)

50

5

50

50

10

50

20

50

50

50

rmEGF (ng/ml)

50

50

50

50

rhWnt-3a (ng/ml)

50

50

25

50

25

50

50

20

rhVEGF121 (ng/ml)

10

rhNeuregulin 1 (nM)

5

rhHGF (ng/ml)

25

Additive

N-2

1

1

1

1

1

 N-21

1

1

1

1

1

1

1

1

1

1

0.5

1

1

1

1

Small Molecular Compounds

Wnt-C59

100

Y-27,632 (µM)

5

10

5

10

10

10

10

10

10

A83-01 (nM)

500

500

500

500

500

500

5000

500

5

5000

500

5000

500

500

500

Dihydrotestosterone (DHT) (nM)

0.1/1

Primocin

1:100v/v

Gastrin I (Human) (nM)

10

10

10

10

10

10

10

Forskolin (µM)

10

10

10

1

Dexamethasone (nM)

10

10

3

SB 202,190

500 nM

10 mM

10 µM

10 µM

1 µM

Prostaglandin E2 (µM)

500

1

Beta-Estradiol (nM)

100

Wnt Surrogate (nM)

0.5

CHIR 99,021 (µM)

0.3

3

Nicotinamide (mM)

10

5

10

10

5

10

10

10

0.1

10

2.5

 N-Acetylcysteine (mM)

1.25

1

1

1.25

1

1

1

1.25

1.25

1.25

1.25

1.25

1

1.25

References

[79]

[82]

[136]

[51]

[50]

[170]

[102]

[171]

[15]

[172]

[149]

[173]

[10]

[174]

[175]

[176]