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Fig. 6 | Experimental Hematology & Oncology

Fig. 6

From: Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cells

Fig. 6

BTYNB inhibits the invasion, migration and proliferation of ESCC cells in vitro. (A) The effects of BTYNB on cell invasion and migration were examined by Transwell assay. 20 µM BTYNB was added to the lower compartment for 24-36 h. (B) Colony formation of KYSE30 and TE1 cells exposed to different concentrations of BTYNB. (C) Cell viability of ESCC cells exposed to BTYNB was determined by CCK-8 assay. KYSE30 and TE1 cells were treated with 5 µM and 10 µM BTYNB for 72 h. (D) Cell apoptosis was determined by flow cytometry. ESCC cells were treated with 10 µM BTYNB for 48 h. (E) Western blotting analysis of the indicated proteins in cells treated with BTYNB. (F) The IGF2BP1-INHBA interaction upon BTYNB treatment (20 µM BTYNB for 24 h) was assessed by RIP-PCR. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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Experimental Hematology & Oncology

ISSN: 2162-3619

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