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Fig. 4 | Experimental Hematology & Oncology

Fig. 4

From: Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cells

Fig. 4

G3BP1 interacts with IGF2BP1. (A) Identification of interacting proteins by Co-IP-MS. The red boxes indicate the differential proteins that were cut off and identified using MS. The whole lane of the IgG group served as a negative control. The table below shows the top eight candidate interacting proteins. (B) Bubble plot of GO enrichment based on mass spectrometry results. (C) The interaction between IGF2BP1 and G3BP1 in ESCC cells was detected with an endogenous immunoprecipitation assay. (D) Cellular localization of endogenously expressed IGF2BP1 (red) or G3BP1 (green) was detected by immunofluorescence staining using laser confocal microscopy. DAPI was used to stain nuclei (blue). Scale bar = 30 μm. (E) Western blotting analysis of the indicated proteins in ESCC cells transfected with NC-siRNA or G3BP1 siRNA. GAPDH was used as a loading control

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Experimental Hematology & Oncology

ISSN: 2162-3619

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