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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cells

Fig. 3

IGF2BP1 enhances ESCC cell invasion and migration by activating INHBA-Smad2/3 signaling. (A) The mRNA levels of IGF2BP1 and INHBA in KYSE30 and TE1 cells after IGF2BP1 knockdown were determined by qRT-PCR. (B-C) The interaction between IGF2BP1 protein and INHBA mRNA in ESCC cells was validated with RIP-PCR (B) and RNA pull-down assay (C). (D) Western blotting analysis of the indicated proteins in ESCC cells transfected with IGF2BP1-specific siRNA or NC siRNA. (E) The decay rate of INHBA mRNA after IGF2BP1 depletion was evaluated by RNA stability assay. (F) Cell lysates were immunoblotted for the indicated proteins after METTL3/14 transient knockdown. (G) m6A modification in INHBA mRNA was tested by gene-specific m6A PCR. (H-I) Cell invasion and migration abilities were examined with Transwell assays. (J) Western blotting analysis of INHBA and Smad2/3 in ESCC cells transfected with INHBA siRNA (above) and in ESCC cells stably expressing shIGF2BP1 transfected with pcDNA3.1-INHBA or empty vector (below). *P < 0.05; ***P < 0.001

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Experimental Hematology & Oncology

ISSN: 2162-3619

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