Fig. 4

DuAb and EDuAb mediate lysis of primary B-ALL cells by health donor T cells. a Representative flow cytometry analysis of the expression of CD19 and CD10 on BMMNCs from six ALL patients. b Representative flow cytometry analysis of residual CD10⁺ tumor cells and remaining CD5⁺ T cells (Left panel). BMMNCs were incubated with health donor T cells (E:T ratio of 5:1) for 72 h at 1 nM concentration of DuAb or EDuAb. Tumor lysis was calculated based on residual CD10⁺ tumor cells (Right panel). (n = 6). c Quantification and statistical analysis of CD69⁺, CD25⁺ and CD107a⁺ cells in health donor T cells after cocultured with B-ALL cells in the presence of 1 nM DuAb or EDuAb. d Quantification of cytokines (IL-2, TNF-α, and IFN-γ) released into culture supernatants by health donor T cells as results of activation and killing tumor cells mediated by DuAb or EDuAb