Fig. 3

DuAb and EDuAb mediate donor primary T cells lysis of CD19 + cell lines in vitro. a Representative flow cytometry analysis showing the expression of CD19 in Nalm6, Namalwa, K562, Nalm6^CD19KO and K562^CD19 cell lines. b Lysis of Nalm6, Namalwa and K562 cells after co-cultured with human primary T cells (E:T ratio of 2:1) in the presence of different concentrations of DuAb and EDuAb for 48 h. c Lysis of Nalm6^CD19KO (Upper panel) and K562^CD19 (Lower panel) cells co-cultured with human primary T cells (E:T ratio of 2:1) in the presence of different concentrations of DuAb and EDuAb for 48 h. d Quantification and statistical analysis of CD69⁺, CD25⁺ and CD107a⁺ cells in human primary T cells after co-cultured with Nalm6 cells in the presence of different concentrations of DuAb or EDuAb. e Quantification of cytokines IL-2, TNF-α, and IFN-γ (from left to right panel) released into culture supernatants by T cells as results of activation and killing tumor cells mediated by DuAb or EDuAb. f Human primary T cells were co-cultured with Nalm6^luciferase cells (E:T ratio of 10:1) at increased concentrations of DuAb or EDuAb for 24 h. The bioluminescence image shown the residual of tumor cells in co-culture system