Fig. 2

The role of DuAb and EDuAb in activating T cells. a Representative flow cytometry analysis of CD25 and CD69 on T cells after stimulated by DuAb and EDuAb (Left panel), quantification and statistical analysis of the data (Right panel). b Cytokines, including IL-2, TNF-α, IFN-γ, release from human primary T cells after stimulated by DuAb or EDuAb for 48 h. c Anti-apoptotic protein BCL-X_L was measured by Western blot. d Proliferation of T cells after stimulated by 1 nM DuAb or EDuAb. e, f Quantification of the apoptosis (early apoptosis: Annexin V⁺ PI⁻; late apoptosis: Annexin V⁺ PI⁺) and PD-1, Lag3 and Tim3 positive T cells after stimulated by DuAb and EDuAb in vitro