Fig. 7

Knockdown of WISP3 blocked Fibronectin-promoted cell migration, invasion, angiogenesis and cancer stemness in NSCLC cells. (A) H1299 cells were transfected with siNC or siWISP3 for 24 h. Then, the migration and invasion ability of the transfected cells treated with or without dish-coated fibronectin were detected by transwell assay. Quantitative analysis of migrated and invaded cells was shown in the histogram. (B) In vitro Matrigel assay was performed to detect the vascular structure formation in H1299 cells treated as (A). (C) Western blot assay was used to detect the proteins of N-catenin, vimentin, OCT4, SOX2 and Nanog in H1299 cells as treated in (A). β-actin was used as a loading control. Quantitative analysis of proteins expression were shown in the histogram. (D) Western blot assay was used to detect the proteins of VEGF, CD31, Tie2 and Ve-cadherin in H1299 cells treated as (A). β-actin was used as a loading control. Quantitative analysis of proteins expression were shown in the histogram. Data were presented by mean ± SD from three independent experiments. *P < 0.05; **P < 0.01 vs. control