Fig. 6

WISP3 promoted tube forming potential of NSCLC cells in vitro. (A) In vitro Matrigel assay was performed to detect the vascular structure formation in H460 and H1299 cells transfected WISP3 overexpression plasmid (pCDH-WISP3) or empty vector (pCDH) for 24 h. (B) H460 and H1299 cells were transfected with pCDH or pCDH-WISP3 for 24 h. Then, Western blot was performed for the detection the proteins of p-β-catenin and β-catenin. β-actin was used as a loading control. Quantitative analysis of p-β-catenin and β-catenin expression were shown in the histogram. (C) H460 and H1299 cells were transfected with pCDH or pCDH-WISP3 for 24 h. Then, Western blot was performed for the detection the proteins of VEGF, CD31, Tie2 and Ve-cadherin. β-actin was used as a loading control. Quantitative analysis of proteins expression were shown in the histogram. Data were presented by mean ± SD from three independent experiments. *P < 0.05; **P < 0.01 vs. control