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Fig. 5 | Experimental Hematology & Oncology

Fig. 5

From: Fibronectin promotes tumor angiogenesis and progression of non-small-cell lung cancer by elevating WISP3 expression via FAK/MAPK/ HIF-1α axis and activating wnt signaling pathway

Fig. 5

WISP3 strengthened cell migration, invasion and colony formation in NSCLC. (A) Correlation between expression of WISP3 and overall survival of lung cancer patients. Data were obtained from the Kaplan-Meier Plotter database. (B) The correlation of WISP3 (CCN6)and fibronectin (FN1) was analyzed using sequencing data from TCGA. (C) Representative IHC staining images for WISP3 in paracancerous tissues (P) and cancer tissues from NSCLC patients. Brown color displays Fibronectin protein levels, with counterstaining by hematoxylin in blue. (D) Western blot was performed to detect the overexpression of WISP3 in H460 and H1299 cells transfected with pCDH or pCDH-WISP3 for 24 h. Quantitative analysis of proteins expression were shown in the histogram. (E) The migration and invasion ability of H460 and H1299 cells transfected with pCDH or pCDH-WISP3 were detected by Transwell assay. Quantitative analysis of migrated and invaded cells was shown in the histogram. (F) Representative photographs of colony formation in H460 and H1299 cells that transfected with pCDH or pCDH-WISP3. The number of colonies was photographed and analyzed in the histogram. Data were presented by mean ± SD from three independent experiments. *P < 0.05; **P < 0.01 vs. control

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Experimental Hematology & Oncology

ISSN: 2162-3619

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