Fig. 4

Fibronectin positively regulated the angiogenesis in NSCLC. (A) In vitro Matrigel assay was performed to detect the vascular structure formation in H460 and H1299 cells treated with or without dish-coated fibronectin (10 µg/mL) for 24 h. (B) H460 and H1299 cells were treated with or without dish-coated fibronectin (10 µg/mL) for 24 h. Then, Western blot was performed for the detection the proteins of VEGF, CD31, Tie2 and Ve-cadherin. β-actin was used as a loading control. Quantitative analysises of proteins expression were shown in the histogram. (C) IHC assay was used to determine the expression of fibronectin and CD31 in tumor tissues from xenograftsderived from H1299 cells mixed with or without fibronectin, and representative data were shown. Data were presented by mean ± SD from three independent experiments. *P < 0.05; **P < 0.01 vs. control