Fig. 3

Fibronectin activated FAK, WNT/β-catenin, MAPK/ERK signaling pathways, and promoted EMT and stem cell properties in NSCLC cells. (A) H460 and H1299 cells were treated with or without dish-coated fibronectin (10 µg/mL) for 24 h. Then, Western blot was performed for the detection the proteins of p-FAK, FAK, p-MEK, MEK, p-ERK and ERK. β-actin was used as a loading control. Quantitative analysis of phosphorylated proteins expression were shown in the histograms. (B) H460 and H1299 cells were treated with or without dish-coated fibronectin (10 µg/mL) for 24 h. Then, t Western blot was performed for the detection the proteins of p-β-catenin and β-catenin. β-actin was used as a loading control. Quantitative analysis of p-β-catenin and β-catenin expression were shown in the histogram. (C) H460 and H1299 cells were treated with or without dish-coated fibronectin (10 µg/mL) for 24 h. Then, Western blot was performed for the detection the proteins of N-cadherin, vimentin, OCT4 and Nanog. β-actin was used as a loading control. Quantitative analysis of proteins expression were shown in the histogram. Data were presented by mean ± SD from three independent experiments. *P < 0.05; **P < 0.01 vs. control