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Fig. 2 | Experimental Hematology & Oncology

Fig. 2

From: Fibronectin promotes tumor angiogenesis and progression of non-small-cell lung cancer by elevating WISP3 expression via FAK/MAPK/ HIF-1α axis and activating wnt signaling pathway

Fig. 2

WISP3 was identified as the candidate target of Fibronectin. (A) Volcano plots, showed genes with partial expression in the control and the dish-coated fibronectin-treated groups in H1299 cells. Red dots showed genes with dramatically higher abundances while the green dots represented genes with significantly lower levels of expression. Values on the x-axis was log2 fold change (log2FC), and the y-axis was log10 p value (log10pvalue). (B) Heat-map of differentially expressed genes from RNA-seq of the control and the fibronectin treated groups. (C and D) Functional analyzing by GO (C) and KEGG (D) of differentially expressed genes in control groups and fibronectin-treated groups. (E) The top 9 differentially expressed genes were subject to qPCR for validation. (F) Western blotting analysis of WISP3 in H460 and H1299 cells treated with or without dish-coated fibronectin (10 µg/mL). Data were presented by mean ± SD from three independent experiments. *P < 0.05; **P < 0.01 vs. control

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Experimental Hematology & Oncology

ISSN: 2162-3619

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