Fig. 1
Fibronectin significantly enhanced metastasis and slightly promoted tumor growth of NSCLC. (A) Representative IHC staining images for Fibronectin in paracancerous tissue (P) and cancer tissues from NSCLC patients. Brown color displays Fibronectin protein levels, with counterstaining by hematoxylin in blue. (B) The proliferation abilities of H460 and H1299 cells treated with or without dish-coated fibronectin (10 µg/mL) for 24 h were measured by MTT assay at the indicated time points. (C) Representative photographs of colony formation in H460 and H1299 cells that treated with or without dish-coated fibronectin (10 µg/mL) for 24 h. The number of colonies was photographed and analyzed in the histogram. (D) The migration abilities of H460 and H1299 cells treated with or without dish-coated fibronectin (10 µg/mL) for 24 h were detected by wound healing assay at 24 and 48 h. (E) The migration and invasion abilities of H460 and H1299 cells treated with or without dish-coated fibronectin (10 µg/mL) for 24 h were detected by Transwell assay. Quantitative analysis of migrated and invaded cells was shown in the histogram. (F) 5 × 106 H1299 cells were suspended in 0.2 ml saline with or without fibronectin (10 µg/mL), and subcutaneously injected into nude mice to generate xenograft model. Three weeks later, all tumors were removed from mice and listed for the photo. Data were presented by mean ± SD for cell experiment, or mean ± SEM animal experiments from three independent experiments. *P < 0.05; **P < 0.01 vs. control