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Fig. 1 | Experimental Hematology & Oncology

Fig. 1

From: Genome-wide CRISPR/Cas9 library screening identified ATM signaling network genes as critical drivers for resistance to ATR inhibition in soft-tissue sarcomas: synthetic lethality and therapeutic implications

Fig. 1

 (A) genome-wide CRISPR/Cas9 negative selection, identification of lost guides corresponding to genes implicated in the resistance to AZD6738 compound. Briefly, for the CRISPR screen with the library GeCKO, 240 millions of IB112 were infected at a MOI = 0.3 and cells were selected during 3 days with puromycin, after selection we let cells grow during approximately one week. For the resistance screen, 30 × 106 cells were treated by DMSO and 30 × 106 cells were treated by 8µM of ATRi. DNA was extracted for each condition, sequenced and compared. (B) Sequences were analyzed with 3 different bioinformatics tools: RIGER, CRISPRcloud2 and Mageck. (C) Genes of interest identified with at least two bioinformatics analysis

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Experimental Hematology & Oncology

ISSN: 2162-3619

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