Fig. 7

Heterogeneity analysis of myeloid cells in primary and lymphatic metastatic TME of PCa. (A). Monocyte distribution subcluster between metastatic and primary lesions using UMAP-2 analysis; (B). Mean percentage of monocyte subclusters in primary and metastatic lesions; (C). Violin plots showing normalized expression levels of marker genes across monocyte subclusters; (D). Metastasis DEGs identified using edgeR package with comparison to primary lesion. Scatter plots showing respective DEG profiles in PCa. Red spots indicate up-regulated genes; green spots indicate no significant change in genes; (E). Functional enrichment analysis for monocyte DEGs; (F). GSEA heatmap of 50 hallmark gene sets in MSigDB database among monocyte subclusters