Fig. 6

The effect of autophagy on HNSCC metastasis in vitro and in vivo. A, B Transwell assay (A) and wound healing assay (B) were used to assess the migration and invasion abilities in FaDu and SCC15 cell lines after overexpression of ANXA6 and dealing with DMSO (10 μm/ml) or CQ (10 μm/ml) for 24 h. C The protein expression levels of Beclin1, LC3, and P62 in HNSCC patients with and without lymphatic metastasis (n = 12). Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; compared with the indicated group