Fig. 4

ANXA6 silencing can inhibit tumor growth and lymphatic metastasis in vivo. A Representative images of HNSCC lymphatic metastasis model (FaDu cells were injected into the foot pads of nude mice and observed for 35 days) for in vivo imaging and fluorescence representing tumorigenesis (n = 5, red color means strong fluorescence, purple means weak). B Representative images of lymphatic metastasis in nude mice. C Representative images of primary tumors and lymph nodes from nude mice (n = 5). D–F. Volume and weight of primary tumor and lymph nodes from nude mice. G Representative images of H&E staining of lymph node metastasis and percentage of metastasis (n = 5). Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; compared with the indicated group