Fig. 1

The expression of ANXA6 in patients with HNSCC. A Heat map shows the mRNA expression levels of differentially expressed genes in three pairs of HNSCC tissues. Red color indicates upregulated, and blue color indicates downregulated. B Relative mRNA expression levels of ANXA6 in HNSCC tissues with and without LM (n = 10). C The protein expression levels of ANXA6 in HNSCC tissues with and without LM and NAT (n = 6). D Representative images of IHC staining of ANXA6 in HNSCC tissues with and without LM, HNSCC tissue stained with PBS instead of primary antibody was as negative control (NC). E Comparisons of overall survival between low ANXA6 expression group and high ANXA6 expression group. F Comparisons of overall survival between LM group and NLM group. LM lymphatic metastasis, NLM non-lymphatic metastasis, NAT normal adjacent tissues. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 ; compared with the indicated group