Fig. 4

Level of surface TREM2 on M-MDSCs being negatively associated with absolute count of CD8⁺ T cells and positively correlated with level of intracellular ARG1 within M-MDSCs. A–C The association between normalized MFI changes of surface TREM2 on PB M-MDSCs and the absolute counts of PB CD8⁺ T cells; the absolute counts of CD8.⁺ T cells in PB being subcategorized by ‘‘low, medium, and high normalized TREM2 levels’’ or “low, intermediate, and high IPI risk scores.” D–F For PB white cells derived from all 100 DLBCL patients, the correlation between the mRNA transcriptional level of TREM2 and ARG1 being calculated by ΔΔCT (Pearson’s R value = 0.843, P < 0.001); mRNA transcriptional levels of TREM2 and ARG1 being further subcategorized by IPI risk scores. G For M-MDSCs isolated from PB of the latest 31 DLBCL patients, the positive correlation between normalized MFI changes of surface TREM2 and intracellular ARG1 utilizing flow-cytometry analysis (Pearson’s R value = 0.738, P < 0.001). ΔΔCT, delta delta cycle threshold; ARG1, arginase 1; HD, healthy donor; IPI, international prognostic index; M-MDSCs, monocytic myeloid-derived suppressor cells; MFI, mean fluorescence intensity; mRNA, messenger ribonucleic acid; n, number; PB, peripheral blood; TREM2, triggering receptors expressed on myeloid cells 2