Fig. 2

LINP1 promotes cell proliferation, migration and invasiveness in cSCC cells. A LINP1 expression was detected after knockout of LINP1 by CRISP/Cas9 technique in HSC-1 cells. B Genomic detections in wildtype and knockout cell strains using primer pair located up- and down-stream of LINP1 locus. Measurements of cell proliferation by CCK-8 assay C, colony formation assay D, transwell migration assay E and Matrigel invasiveness measurement F were performed in HSC-1 cells treated with siRNAs targeting LINP1. G LINP1 expression was detected by qPCR after overexpression of LINP1 in HSC-1 cells. Measurements of cell proliferation by CCK-8 assay H, colony formation assay I, transwell migration assay J and Matrigel invasiveness measurement K were performed in HSC-1 cells overexpressing LINP1. Scale bars, 500 mm C, I, 100 μm E, F, J, K. Each experiment was performed in at least triplicate and results are presented as mean ± s.d. One-way ANOVA and Dunnett’s multiple comparison test were used to analyze the data (*P < 0.05, **P < 0.01, ***P < 0.001)