Fig. 4

ASAP2 facilitates hepatocellular carcinoma (HCC) migration and invasion. A Associations between migration/invasion-related molecular signatures and ASAP2 expression in The Cancer Genome Atlas (TCGA) Liver hepatocellular carcinoma (LIHA) cohort according to gene set enrichment analysis (GSEA). B Metastasis-related molecular signatures were enriched in ASAP2-high HCC samples according to TCGA dataset. C Effects of ASAP2 knockdown on the migration rates of SNU182 (left) and SNU387 (right) cells were assessed by wound healing experiments. D Effects of ASAP2 overexpression on the migration rates of Hep3B cells were assessed by wound healing experiments. E Effects of ASAP2 knockdown on the invasion capacity of SNU182 (left) and SNU387 (right) cells were assessed by Transwell experiments. F Effects of ASAP2 overexpression on the invasion capacity of Hep3B cells were assessed by Transwell experiments. G shControl or shASAP2 SNU182 cells were injected into recipient mice through the tail vein to establish lung metastasis models; Representative images of the metastases are illustrated in the left panel and the incidence of lung metastases are shown in the right panel