Fig. 4

p53 activation could downregulate CDC20 expression. A GSE10793 (n = 66), GSE93291 (n = 123) and patients in our cohort (n = 24) showed that TP53 expression was negatively correlated with CDC20 expression. B The mRNA expression level of TP53, P21 and CDC20 in Jeko, Mino, Z138 and JVM2 cells were detected by RT-qPCR after treatment with 5 μM (Z138 and JVM2) or 10 μM (Jeko and Mino) nutlin-3a for 24 h. C The protein expression level of p53, p21 and CDC20 in Jeko, Mino, Z138 and JVM2 cells were analyzed by WB after treatment with 5 μM (Z138 and JVM2) or 10 μM (Jeko and Mino) nutlin-3a for 24 h. The increase in p53 expression was accompanied by a decrease in CDC20 expression only in Z138 and JVM2 cells (B, C). Data were obtained from at least three independent experiments and presented as mean ± SD. *P < 0.05, ***P < 0.001 compared with the control group, ns meant P > 0.05