Fig. 1

A novel TPR-PDGFRB fusion gene was identified in one patient with Ph-like ALL. A Graphical representation of the organization process for the TPR-PDGFRB fusion at the chromosome level. B Diagram of the whole treatment process of the TPR-PDGFRB positive patient. NR, not remission; PR: partial remission; CR: complete remission; MRD: minimal residual disease. C Interphase FISH analysis with PDGFRB break-apart probe showing a split PDGFRB signal pattern in primary and CNS-L blasts. The arrow indicates a break-apart signal in the PDGFRB gene. D RT-PCR was performed across the TPR-PDGFRB fusion gene breakpoint, and a 423-bp product from the forward primer (5′-AGTCTGTAGGACGTGGCCTT-3′) located in the exon 44 of TPR gene and reverse primer (5′-TGGGGTCCACGTAGATGTACTC-3′) located in the exon 12 of PDGFRB gene was amplified. E Schematic diagrams of the TPR, PDGFRB, and TPR-PDGFRB fusion proteins. The breakpoint is indicated by a red dashed line. TM: transmembrane domain. F, NIH 3T3 cells, TPR-PDGFRB fusion-negative B-ALL cells, and primary blasts were immunoblotted for TPR (Abcam #ab70610) and PDGFRB (CST, #3169)