Fig. 4

Effects of E. coli, S. aureus and LPS on neutrophils in WT and GPR109A^−/− mice. Neutrophils from WT and GPR109A^−/− mice were stimulated by E. coli, S. aureus and LPS, respectively. NE, MPO and H3 in NETs were stained by immunofluorescence. a–c Immunofluorescence staining results of NE, MPO and H3. All results were observed under laser confocal microscope