Fig. 10

Effect of GPR109A on Cit-H3 in liver, spleen, lung and kidney. The liver, spleen, lung and kidney of WT mice and GPR109A^−/− mice in CLP model were collected and extracted the total protein. Then the expression level of Cit-H3 in liver, spleen, lung and kidney was detected by western blotting. a–d Expression level of Cit-H3 in liver, spleen, lung and kidney. e Neutrophils can enter tissues through blood circulation and release NETs in tissues to play a bactericidal function. Cit-H3 is an important marker of NETs formation. Values are presented as means ± SEM (n = 10) (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001)