Fig. 3

Cytotoxic properties of CD26^neg/low/high CD8⁺ T cells in CLL. A Representative flow plot of the gating strategy for CD26^neg, CD26^low, and CD26^high CD8⁺ T cells. B Representative flow plots of the co-expression of Granzyme-B (GzmB)/Perforin in CD26^neg/low/high CD8⁺ T cells in CLL. C Cumulative data showing the frequency of GzmB⁺, (D) Perforin⁺, and (E) GzmB⁺Perforin⁺ cells among CD26^neg/low/high CD8⁺ T cells in CLL. F Representative flow plots, and (G) cumulative data of the frequency of GzmB⁺GzmK⁺ cells among CD26^neg/low/high CD8⁺ T cells in CLL. H Representative flow plots, and (I) cumulative data of the frequency of CD107a⁺ cells among CD26^neg/low/high CD8⁺ T cells in CLL either unstimulated (unstim) or stimulated (stim) with anti-CD3/CD28 (3 g\(\upmu\)/ml, 1 g\(\upmu\)/ml) in the presence of protein transport inhibitor (1/1000). J Representative flow plots, and cumulative data of the frequency of (K) GzmB⁺ (L) GzmK⁺, and (M) GzmB⁺GzmK⁺ cells among CD26^neg/low/high CCR7⁻CD8⁺ T cells in CLL either unstimulated or stimulated with anti-CD3/CD28(3 g\(\upmu\)/ml, 1 g\(\upmu\)/ml), and a cocktail of IL-18 + IL-12 + IL-15 (100 ng/ml of each). Error bars represent median with interquartile range. Each dot represents an individual human sample