Fig. 6
Irradiation rescues ecto-CRT and sensitizes B16F10 tumor cells to ICB in vivo. a A single dose of 20 Gy irradiation treatment affected cell surface CRT expression in pre-treated B16F10 cells. b–e B16-C8KO or control cells (2 × 105) were subcutaneously inoculated into the right legs of C57BL/6 mice. When the tumors reached approximately 50 mm3, the mice were locally irradiated with a single dose of 20 Gy. On the same day, 200 µg of anti-PD-1 monoclonal antibody or the equivalent isotype control antibody was injected intraperitoneally every three days three times. b Scheme of irradiation combined with anti-PD-L1 treatment for the B16F10 xenograft mouse model, and tumor growth curves of the indicated groups (c) and each tumor (d). e Overall survival in the indicated groups. When tumor volumes reached 2000 mm3, the mice were sacrificed